Genome Differentiation in Lycoris Species (Amaryllidaceae) Identified by Genomic in situ Hybridization

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概要

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• The chromosomes of six Lycoris species and three interspecific hybrids were investigated using genomic in situ hybridization (GISH) to clarify the chromosome constitution in this genus. Total DNA from L. aurea (2n=13, 9M+4T) and L. sprengeri (2n=22, 22A) was used as probe and blocking DNA, respectively. All the chromosomes of L. aurea (9M+4T) and L. longituba (6M+10T) were hybridized with probe DNA and were visualized as yellow labeled chromosomes, while those of L. sprengeri (22A), L. radiata var. pumila (22A) and L. sanguinea (22A) remained unlabeled and appeared as red chromosomes. Eight labeled (3M+5T) and 22 unlabeled chromosomes (20A+1M'+1m) were observed in GISH of L. incarnata. Eight labeled (3M+5T) and 33 unlabeled chromosomes (31A+1M'+1m) were detected in GISH of three interspecific hybrids between L. incarnata and 3 diploid species. The distinction between M+T and A type chromosomes at the DNA sequence level by GISH demonstrated that genome differentiation had occurred in the genus Lycoris.

• 日本育種学会の論文

著者

• Ogawa Takeshi

  Graduate School Of Life And Environmental Sciences Osaka Prefecture University

• Kurita Siro

  Department Of Biology Faculty Of Science Chiba University

• Ueno Mikako

  Graduate School Of Life And Environmental Sciences Osaka Prefecture Univ.

• Ma Biao

  Laboratory Of Genetics And Plant Breeding Graduate School Of Agriculture And Biological Sciences Osa

• Ma Biao

  Graduate School Of Life And Environmental Sciences Osaka Prefecture University

• Tarumoto I

  Graduate School Of Agriculture And Life Sciences Osaka Prefecture University

• Tarumoto Isao

  Laboratory Of Genetics And Plant Breeding Graduate School Of Agriculture And Biological Sciences Osa

• OGAWA Takeshi

  Laboratory of Plant Molecular Improvement and Global COE Program, Graduate School of Nutritional and

• Ueno Mikako

  Laboratory of Genetics and Plant Breeding, Graduate School of Agriculture and Biological Sciences, O

• Ogawa Takeshi

  Laboratory Of Plant Molecular Improvement And Global Coe Program Graduate School Of Nutritional And

• OGAWA Takeshi

  Department of Pure and Applied Sciences, University of Tokyo

• OGAWA Takeshi

  Department of Pure and Applied Sciences University of Tokyo

• OGAWA Takeshi

  Laboratory of Genetics and Plant Breeding, Graduate School of Agriculture and Biological Sciences, Osaka Prefecture University

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