Studies on Speciation in Genus <I>Lycoris</I> Using Interspecific Hybrids and Selfed Plants Produced through Embryo Rescue
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概要
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This study was carried out to overcome the problems that impede genetic improvement of <I>Lycoris</I>. An improved ovule culture method was developed to rescue the abortive embryo in order to overcome the poor fertility in hybridization of <I>Lycoris</I>. Using this method, a large number of selfed and crossed plants in several species were produced, and the interspecific hybrids which used <I>L. incarnata </I>(sterile) as a cross parent were obtained for the first time. Cytological studies of the S<SUB>1</SUB> plants indicated that polyploidization and aneuploid reduction caused by self-pollination should be among the major factors affecting karyotype evolution in some <I>Lycoris </I>species. Allozyme segregations in polymorphic loci of APT, GOT and EST were observed in S<SUB>1</SUB> of five diploid species, suggesting that each of the species is originally heterozygous. The investigation using genomic <I>in situ </I>hybridization (GISH) was done to clarify the chromosome constitution in <I>Lycoris</I>. The distinction between M + T and A type chromosomes at the DNA sequence level demonstrated that genome differentiation has occurred in the genus <I>Lycoris.</I>
- 独立行政法人 国際農林水産業研究センターの論文
著者
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Ma Biao
Laboratory Of Genetics And Plant Breeding Graduate School Of Agriculture And Biological Sciences Osa
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Tarumoto Isao
Laboratory Of Genetics And Plant Breeding Graduate School Of Agriculture And Biological Sciences Osa
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OGAWA Takeshi
Laboratory of Genetics and Plant Breeding, Graduate School of Agriculture and Biological Sciences, Osaka Prefecture University
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MA Biao
Laboratory of Genetics and Plant Breeding, Graduate School of Agriculture and Biological Sciences, Osaka Prefecture University
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