Hepatic expression of spermatogenic genes and their transiently remarkable downregulations in Wistar-Kyoto rats in response to lead-nitrate administration : strain-difference in the gene expression patterns
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概要
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Administration of lead ion (Pb) to rats and mice affects hepatic functions such as the induction of hepatic cell proliferation and upregulation of cholesterol biosynthesis. To identify the genes for which expression changes in response to Pb-administration, we analyzed hepatic gene expression patterns in stroke-prone spontaneously hypertensive rat (SHRSP), its normotensive control, Wistar-Kyoto rat (WKY), and Spraque-Dawley (SD) rat strains, 3, 6, and 12 hr later after single i.v. injection of lead nitrate (LN) at a dose of 100 µmol using a DNA microarray technique. The data analysis demonstrated that the expression of a great number of genes was transiently and remarkably downregulated 3 hr after LN-injection, and then recovered to control levels only in LN-injected WKY. These normal hepatic expression levels in WKY and SHRSP were much higher than those in SD rats. Furthermore, most of these genes were ones thought to be expressed specifically in the spermatids and/or testes; i.e. genes encoding protamin 1, transition protein 1, and transition protein 2. These findings suggest that the regulation system common to expression of all of these genes could be a target site of Pb-toxic action, at least, in the liver of WKY, and that this system might be similar to the system essential for spermatogenesis, especially spermiogenesis, in the testis. In addition, it appears that clarifying the cause of the difference between the systems of WKY and SHRSP might aid in identifying the pathologic genes in SHRSP. Finally, it will be an important to clarify how the products of the genes related to spermatogenesis, including spermiogenesis, are functional in the livers of WKY and SHRSP.
- 2011-06-01
著者
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NEMOTO Kiyomitsu
Department of Molecular Toxicology and COE Program in the 21st Century, School of Pharmaceutical Sci
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Kojima Misaki
Animal Genome Research Unit Division Of Animal Sciences National Institute Of Agrobiological Science
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Ito Sei
Department Of Bioscience And Biotechnology Graduate School Of Bioresource And Bioenvironmental Scien
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Degawa M
Department Of Molecular Toxicology And Global Coe Program School Of Pharmaceutical Sciences Universi
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Degawa Masakuni
Department Of Hygienic Chemistry Faculty Of Pharmaceutical Sciences Tohoku University
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Nemoto Kiyomitsu
Department Of Molecular Toxicology And Global Coe Program School Of Pharmaceutical Sciences Universi
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YOSHIDA Chiaki
Department of Molecular Toxicology and Global COE Program, School of Pharmaceutical Sciences, Univer
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MIYATA Misaki
Department of Molecular Toxicology and Global COE Program, School of Pharmaceutical Sciences, Univer
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Miyata Misaki
Department Of Molecular Toxicology And Global Coe Program School Of Pharmaceutical Sciences Universi
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Yoshida Chiaki
Department Of Molecular Toxicology And Global Coe Program School Of Pharmaceutical Sciences Universi
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Degawa Masakuni
Department Of Molecular Toxicology And Global Coe Program School Of Pharmaceutical Sciences Universi
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Kojima Misaki
Animal Genome Res. Unit Div. Of Animal Sciences National Inst. Of Agrobiological Sciences
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Kojima Misaki
Animal Genome Research Unit Division Of Animal Sciences National Institute Of Agrobiological Science
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Ito Sei
Department Of Molecular Toxicology And Global Coe Program School Of Pharmaceutical Sciences Universi
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Degawa Masakuni
Dep. Of Molecular Toxicology And Global Center Of Excellence Program School Of Pharmaceutical Scienc
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Miyata Misaki
Department of Endocrinology and Diabetes, Nagoya Ekisaikai Hospital, Japan
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