Establishment of a Rat Hepatic Cell Line, KanR2-XL8, for a Reporter Gene Assay of Aryl Hydrocarbon Receptor Ligands
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概要
- 論文の詳細を見る
To establish a highly sensitive and convenient reporter gene assay for aryl hydrocarbon receptor (AhR) ligands, the chimera plasmid xenobiotic responsible element-luciferase (XRE-Luc) containing an XRE, minimal SV40 promoter, and luciferase reporter gene, was first constructed. The XRE-Luc and the expression vector pRC/CMV containing a neomycin-resistant gene were transfected into a rat hepatic cell line, Kan-R2, and then KanR2-XL8 was selected as a cell clone, which showed the highest response to the induction of luciferase by aryl hydrocarbons, 3-methylcholanthlene (3-MC) and benzo[a]pyrene. Furthermore, AhR-regulated genes, CYP1A1 and CYP1B1, in KanR2-XL8 cells were also activated by 3-MC. In the present study, we established a hepatic cell line, KanR2-XL8, that is useful for screening of AhR ligands with two parameters, the activations of the transfected luciferase gene and the AhR-regulated genes in a host cell.
- 公益社団法人日本薬学会の論文
- 2004-10-01
著者
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DEGAWA Masakuni
Department of Molecular Toxicology, School of Pharmaceutical Sciences, University of Shizuoka
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NEMOTO Kiyomitsu
Department of Molecular Toxicology and COE Program in the 21st Century, School of Pharmaceutical Sci
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SEKIMOTO Masashi
Department of Molecular Toxicology and COE Program in the 21st Century, School of Pharmaceutical Sci
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Miyajima S
Department Of Environmental Health Faculty Of Pharmaceutical Sciences Hokuriku University
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Sato M
Section Of Genetic Engineering Research Center For Genetic Engineering And Cell Transplantation Toka
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Sekimoto Masashi
Department Of Molecular Toxicology And Global Coe Program School Of Pharmaceutical Sciences Universi
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Degawa M
Department Of Molecular Toxicology And Global Coe Program School Of Pharmaceutical Sciences Universi
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Degawa Masakuni
Department Of Hygienic Chemistry Faculty Of Pharmaceutical Sciences Tohoku University
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MIYAJIMA Shoji
Department of Molecular Toxicology and COE Program in the 21st Century, School of Pharmaceutical Sci
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Iwamoto Miho
Department of Molecular Toxicology and COE Program in the 21st Century, School of Pharmaceutical Sci
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Iwamoto Miho
Department Of Molecular Toxicology And Coe Program In The 21st Century School Of Pharmaceutical Scie
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Nemoto Kiyomitsu
Department Of Molecular Toxicology And Global Coe Program School Of Pharmaceutical Sciences Universi
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Degawa Masakuni
Department Of Molecular Toxicology And Global Coe Program School Of Pharmaceutical Sciences Universi
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Degawa Masakuni
Dep. Of Molecular Toxicology And Global Center Of Excellence Program School Of Pharmaceutical Scienc
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Miyajima Shoji
Department of Molecular Toxicology and COE Program in the 21st Century, School of Pharmaceutical Sciences, University of Shizuoka
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