Decreased Gene Expression of Testicular Cell-Specific Proteins in Cadmium-Induced Acute Testicular Toxicity

スポンサーリンク

概要

• 論文の詳細を見る

• Cadmium salts induce severe acute testicular necrosis in rodents. We assessed the expression levels of the genes encoding the follicle-stimulating hormone receptor, luteinizing hormone receptor, testis-specific histone 2B, and transition proteins 1 and 2, which are preferentially expressed in Sertoli cells, Leydig cells, spermatocytes, and spermatids, respectively, by reverse transcription (RT)-PCR using total RNA prepared from the whole testes of cadmium chloride (Cd)-administered rats. Spraque Dawley rats at 3, 7, and 12 weeks of age were singly and subcutaneously injected with Cd at doses of 1, 2.5, 5, 10, 15 or 20 μmol/kg. The expression levels of all genes tested were significantly decreased at doses of over 15 μmol/kg (3-week-old rats) or over 10 μmol/kg (7- and 12-week-old rats) 96 hr after injection. Histopathological study showed that these dosages of Cd resulted in extensive disruption of the seminiferous tubules and necrosis of testicular cells, while administration of Cd at a dose of 5 μmol/kg in 7- and 12-week-old rats resulted in only partial degeneration of seminiferous tubules and testicular cells. Therefore, their reduced gene expression is likely to serve as an indicator of Cd-induced testicular necrosis accompanied by cell death. In addition, the susceptibility to Cd-induced testicular damage and decreased gene expression was higher in mature (7- and 12-week-old) rats than in immature (3-week-old) rats.

• 公益社団法人　日本薬学会の論文

著者

• DEGAWA Masakuni

  Department of Molecular Toxicology, School of Pharmaceutical Sciences, University of Shizuoka

• NEMOTO Kiyomitsu

  Department of Molecular Toxicology and COE Program in the 21st Century, School of Pharmaceutical Sci

• SEKIMOTO Masashi

  Department of Molecular Toxicology and COE Program in the 21st Century, School of Pharmaceutical Sci

• Miyajima S

  Department Of Environmental Health Faculty Of Pharmaceutical Sciences Hokuriku University

• YAMADA Masaki

  Department of Applied Chemistry. Faculty of Engineering, Toyama University

• Degawa Masakuni

  Department Of Hygienic Chemistry Faculty Of Pharmaceutical Sciences Tohoku University

• MIYAJIMA Shoji

  Department of Molecular Toxicology and COE Program in the 21st Century, School of Pharmaceutical Sci

• Hara Shiori

  Department of Molecular Toxicology and Global Center of Excellence (COE) Program, School of Pharmace

• Saigusa Ryosuke

  Department of Molecular Toxicology and Global Center of Excellence (COE) Program, School of Pharmace

• Shikama Hiroshi

  Central Research Institute, Ishihara Sangyo Kaisha, Ltd.

• Yotsuya Shuichi

  Central Research Institute, Ishihara Sangyo Kaisha, Ltd.

• Yotsuya Shuichi

  Central Research Institute Ishihara Sangyo Kaisha Ltd.

• Nemoto Kiyomitsu

  Department Of Molecular Toxicology And Global Coe Program School Of Pharmaceutical Sciences Universi

• Shikama Hiroshi

  Central Research Institute Ishihara Sangyo Kaisha Ltd.

• Degawa Masakuni

  Department of Molecular Toxicology and Global Center of Excellence (COE) Program, School of Pharmaceutical Sciences, University of Shizuoka

• Nemoto Kiyomitsu

  Department of Molecular Toxicology and Global Center of Excellence (COE) Program, School of Pharmaceutical Sciences, University of Shizuoka

• Miyajima Shoji

  Department of Molecular Toxicology and Global Center of Excellence (COE) Program, School of Pharmaceutical Sciences, University of Shizuoka

• Hara Shiori

  Department of Molecular Toxicology and Global Center of Excellence (COE) Program, School of Pharmaceutical Sciences, University of Shizuoka

• Yamada Masaki

  Department of Acooustic Design,Kyushu Institute of Design

関連論文

• The Effects of D-galactosamine- or Carbon Tetrachloride-Induced Regeneration on Induction of Rat Liver Cell Foci in a Model for Detection of Initiation Activities of Chemicals
• Selective Increase in Decorin Core mRNA Level in Cultured Vascular Smooth Muscle Cells after Exposure to Advanced Glycation Endproducts
• Induction of Decorin Core Protein Synthesis by Advanced Glycation Endproducts in Cultured Vascular Smooth Muscle Cells(PROCEEDINGS OF 24TH SYMPOSIUM ON TOXICOLOGY AND ENVIRONMENTAL HEALTH)
• Remodeling of Vascular Endothelial Glycocalyx by Basic Fibroblast Growth Factor (Proceedings of the 23rd Symposium on Toxicology and Environmental Health)
• Augmentation of 3-methylcholanthrene-induced bioactivation in the human hepatoma cell line HepG2 by the calcium channel blocker nicardipine
• Decreased Levels of 2-Amino-3-methylimidazo[4,5-f]quinoline-DNA Adducts in Rats Treated with β-Carotene, α-Tocopherol and Freeze-dried Aloe
• The gene expression of hepatic proteins responsible for DNA repair and cell proliferation in tamoxifen-induced hepatocarcinogenesis
• No Involvement of the Nerve Growth Factor Gene Locus in Hypertension in Spontaneously Hypertensive Rats
• A hepatocarcinogenic tryptophan-pyrolyzate component, Trp-P-1, decreases serum total testosterone level and induces hepatic Cyp1a2 in male mice
• Neurotrophin-3 gene expression negatively responds to serum through AP-1 transcription factor in rat cultured vascular smooth muscle cells
• A Thermolytic Azulene Synthesis from Dimethyl 4-Morpholino-3,3a,8,8a-tetrahydroazulene-5,6-dicarboxylates and Its 1-Substituted Derivatives
• Synthesis and Properties of Azuleno[1,2-a]acenaphthylene and Dimethyl acenaphthyleno[1,2-d]heptalene-8,9-dicarboxylate
• "Highly Destabilized Carbocations," Generation and Rearrangements in the Reactino of 8,9,10,11-Tetrahydrocyclohepta[a]phenalen-6(12H)-one-12-ol and -7(12H)-one-12-ol with Sulfuric Acid
• Effects of Lead Nitrate on Liver Weight and Serum Total Cholesterol Amounts in Stroke-Prone Spontaneously Hypertensive and Wistar-Kyoto Rats
• SUSCEPTIBILITY OF ANIMALS TO HEPATOCARCINOGENIC AROMATIC AMINES CORRELATES WITH THE INDUCTION OF THE CARCINOGEN ACTIVATION ENZYME (S) WITH THE AMINES
• O11-10 Changes in the expression of the genes related to chemical carcinogenesis during tamoxifen-induced hepatocarcinogenesis in rats
• DNA damage and altered gene expression of enzymes for metabolism and DNA repair by tamoxifen and toremifene in the female rat liver
• Increased Expression of Cyclin-Dependent Kinase-Interacting Protein p21 during Tamoxifen-Induced Hepatocarcinogenesis in Female Rats
• Dysfunction of Paracellin-1 by Dephosphorylation in Dahl Salt-Sensitive Hypertensive Rats
• INDUCTION OF HEPATIC CYTOCHROME P450 ISOFORMS BY NICARDIPINE AT THERAPEUTIC DOSES IN SPONTANEOUSLY HYPERTENSIVE RATS
• The Induction of Hepatic Cytochrome P4501A Subfamily Enzymes by Nitroanisidines : Species Difference among Experimental Rodents
• Immobilization stress reduced the expression of neurotrophins and their receptors in the rat brain
• Establishment of a Human Hepatoma Cell Line HepG2-A10 for a Reporter Gene Assay of Arylhydrocarbon Receptor Activators
• Establishment of a Rat Hepatic Cell Line, KanR2-XL8, for a Reporter Gene Assay of Aryl Hydrocarbon Receptor Ligands
• Gender-Related Differences in the Level of Serum Lipids in Meishan Pigs
• Constitutive Expression of Cytochrome P450 Genes in Newly Established Rat Hepatic Cell Lines
• Decreased Gene Expression of Testicular Cell-Specific Proteins in Cadmium-Induced Acute Testicular Toxicity
• Fine Linkage Mapping of the Blood Pressure Quantitative Trait Locus Region on Rat Chromosome 1
• Effect of IS-741 on Cell Adhesion between Human Umbilical Vein Endothelial Cells and HL-60 Cells
• Changes in expression of hepatic cytochrome P450 subfamily enzymes during development of adjuvant-induced arthritis in rats
• Efficacy of the Inflammatory Cell Infiltration Inhibitor IS-741 on Colitis Induced by Dextran Sulfate Sodium in the Rat
• Interleukin-1 Controls the Constitutive Expression of the Cyp7a1 Gene by Regulating the Expression of Cyp7a1 Transcriptional Regulators in the Mouse Liver
• Altered expression of GADD45 genes during the development of chemical-mediated liver hypertrophy and liver tumor promotion in rats
• Super-induced gene expression of the N-methyl-D-aspartate receptor 2C subunit in chemical-induced hypertrophic liver in rats
• ALTERATION OF HEPATIC CYTOCHROME P-450 ISOZYMES DURING HEPATOCARCINOGENESIS PROCESS IN RATS
• Altered Expression of Hepatic CYP1A Enzymes in Rat Hepatocarcinogenesis
• Hepatic expression of spermatogenic genes and their transiently remarkable downregulations in Wistar-Kyoto rats in response to lead-nitrate administration : strain-difference in the gene expression patterns
• Oxidative DNA Damage Induced by an N-Hydroxy Metabolite of Carcinogenic 4-Dimethylaminoazobenzene
• Gene Activations of CYP2B1 and CYP3A1 by Dihydropyridine Calcium Channel Antagonists in the Rat Liver : the Structure-Activity Relationship(Biochemistry/Molecular Biology)
• Influence of the shape and size of a dummyhead upon thevenin acoustic impedance and Thevenin pressure
• Altered expression of GADD45 genes during the development of chemical-mediated liver hypertrophy and liver tumor promotion in rats
• Super-induced gene expression of the N-methyl-D-aspartate receptor 2C subunit in chemical-induced hypertrophic liver in rats
• Estrogen and androgen receptor status in hepatocellular hypertrophy induced by phenobarbital, clofibrate, and piperonyl butoxide in F344 rats
• Inhibitory Effects of Citrus Polymethoxyflavones, Nobiletin and its Analogues, on Acetylcholinesterase Activity
• 9-Methyl-7-bromoeudistomin D, a Caffeine Like Ca^ Releaser, Probably Binds to Cytochromes P450 1A1 and 2B in Liver Microsomes
• Establishment of a Stable Human Cell Line, HPL-A3, for Use in Reporter Gene Assays of Cytochrome P450 3A Inducers
• Involvement of Interleukin-1 in Lead Nitrate-Induced Hypercholesterolemia in Mice
• Comparison of constitutive gene expression levels of hepatic cholesterol biosynthetic enzymes between Wistar-Kyoto and stroke-prone spontaneously hypertensive rats
• The synthesis and properties of cyclohepta(a)phenalene-6,10-, -6,12-, -7,10-, and -7,12-diones and thier dicationic and dianionic species.
• Optical resolution of .BETA.-hydroxynorvaline.
• Electronic Spectra of Phenanthrolinediones, Phenanthrolinones, Bipyridinediones, and Amino-Substituted Phenanthrolines.
• Synthesis of 2,9-dichloro-1,10-phenanthroline from N,N'-annelated phenanthrolinediones.
• Induced expression of hepatic N-methyl-D-aspartate receptor 2C subunit gene during liver enlargement induced by lead nitrate, a hepatocellular mitogen
• Upregulation of N-methyl-D-aspartate receptor subunits and c-Fos expressing genes in PC12D cells by nobiletin
• Change in the gene expression of the N-methyl-D-aspartate receptor 2C subunit by dietary β-naphthoflavone, indole-3-carbinol, or acetaminophen in the rat liver

もっと見る 閉じる

スポンサーリンク