Comparative Enzymatic Analysis of Azoreductases from Bacillus sp. B29
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概要
- 論文の詳細を見る
We cloned and expressed two genes encoding azoreductase homologes, AzrB and AzrC, from Bacillus sp. B29. Purified recombinant AzrB and AzrC were homodimers with 23 kDa identical subunits, and were flavoproteins. NADH was preferred as electron donors for both azoreductases. The azoreductases showed optimal activities at 70 °C (AzrB) and 55 °C (AzrC), and retained activities up to 55 °C (AzrB) and 50 °C (AzrC) after incubation for 1 h. Other enzymatic properties, including the substrate specificities of both azoreductases, were also investigated.
- 社団法人 日本農芸化学会の論文
- 2009-05-23
著者
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SHIBATA Takeshi
Division of Electrical, Electric and Information Engineering, Graduate School of Engineering, Osaka
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Matsumoto Ken'ichiro
Division Of Biotechnology And Macromolecular Chemistry Graduate School Of Engineering Hokkaido Unive
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TAGUCHI Seiichi
Division of Biotechnology and Macromolecular Chemistry, Graduate School of Engineering, Hokkaido Uni
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Ooi Toshihiko
Division of Biotechnology and Macromolecular Chemistry, Graduate School of Engineering, Hokkaido Uni
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Taguchi Seiichi
Division Of Biotechnology And Macromolecular Chemistry Graduate School Of Engineering Hokkaido Unive
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KINOSHITA Shinichi
Division of Biotechnology and Macromolecular Chemistry, Graduate School of Engineering, Hokkaido Uni
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Kinoshita Shinichi
Division Of Biotechnology And Macromolecular Chemistry Graduate School Of Engineering Hokkaido Unive
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Taguchi Seiichi
Division Of Biotechnology And Macromolecular Chemistry Graduate School Of Engineering Hokkaido Unive
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Matsumoto Ken'ichiro
Division Of Biotechnology And Macromolecular Chemistry Graduate School Of Engineering Hokkaido Unive
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Shibata Takeshi
Division Of Biotechnology And Macromolecular Chemistry Graduate School Of Engineering Hokkaido Unive
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Morimoto Kenjiro
Division Of Biotechnology And Macromolecular Chemistry Graduate School Of Engineering Hokkaido Unive
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Ooi Toshihiko
Division Of Biotechnology And Macromolecular Chemistry Graduate School Of Engineering Hokkaido Unive
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Taguchi Seiichi
Division Of Biotechnology And Macromolecular Chemistry Graduate School Of Engineering Hokkaido Unive
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