Improved polyhydroxybutyrate (PHB) production in transgenic tobacco by enhancing translation efficiency of bacterial PHB biosynthetic genes(PLANT BIOTECHNOLOGY)

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概要

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• Polyhydroxybutyrate [P(3HB)] was produced in the transgenic tobacco harboring the genes encoding acetoacetyl-CoA reductase (PhaB) and polyhydroxyalkanoate synthase (PhaC) from Ralstonia eutropha (Cupriavidus necator) with optimized codon usage for expression in tobacco. P(3HB) contents in the transformants (0.2mg/g dry cell weight in average) harboring the codon-optimized phaB gene was twofold higher than the control transformants harboring the wild-type phaB gene. The immunodetection revealed an increased production of PhaB in leaves, indicating that the enhanced expression of PhaB was effective to increase P(3HB) production in tobacco. In contrast, codon-optimization of the phaC gene exhibited no apparent effect on P(3HB) production. This result suggests that the efficiency of PhaB-catalyzed reaction contributed to the flux toward P(3HB) biosynthesis in tobacco leaves.

著者

• Shimada Hiroaki

  Department Of Biological Science And Technology Tokyo University Of Science

• Matsumoto Ken'ichiro

  Division Of Biotechnology And Macromolecular Chemistry Graduate School Of Engineering Hokkaido Unive

• Taguchi Seiichi

  Division Of Biotechnology And Macromolecular Chemistry Graduate School Of Engineering Hokkaido Unive

• Taguchi Seiichi

  Division Of Biotechnology And Macromolecular Chemistry Graduate School Of Engineering Hokkaido Unive

• Matsumoto Ken'ichiro

  Division Of Biotechnology And Macromolecular Chemistry Graduate School Of Engineering Hokkaido Unive

• Morimoto Kenjiro

  Division of Biotechnology and Macromolecular Chemistry, Graduate School of Engineering, Hokkaido Uni

• Gohda Aoi

  Division of Biotechnology and Macromolecular Chemistry, Graduate School of Engineering, Hokkaido Uni

• Shimada Hiroaki

  Department Of Biological Science And Technology Tokyo University Of Science:research Institute For S

• Gohda Aoi

  Division Of Biotechnology And Macromolecular Chemistry Graduate School Of Engineering Hokkaido Unive

• Morimoto Kenjiro

  Division Of Biotechnology And Macromolecular Chemistry Graduate School Of Engineering Hokkaido Unive

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