Morphological Change in Cellular Granule Formation of Poly[(R)-3-hydroxybutyrate] Caused by DNA-Binding-Related Mutations of an Autoregulated Repressor PhaR
スポンサーリンク
概要
- 論文の詳細を見る
PhaP is a major poly[(R)-3-hydroxybutyrate] [P(3HB)]-granule-associated protein. Its gene expression is controlled by an autoregulated repressor, PhaR, in Paracoccus denitrificans. The packing force of the P(3HB) granule by PhaP is greatly influenced by the number of PhaP molecules. In this study, the effects of DNA-binding-ability-reduced mutations of PhaR on morphological change in the cellular granule formation of P(3HB) were examined under a transmission electron microscope using an Escherichia coli recombinant system. Microscopic observation indicated that stronger packing of P(3HB) granules took place when the number of PhaP molecules was increased by reduction in the DNA-binding ability of PhaR.
- 社団法人 日本農芸化学会の論文
- 2007-06-23
著者
-
Taguchi Seiichi
Division Of Biotechnology And Macromolecular Chemistry Graduate School Of Engineering Hokkaido Unive
-
Yamada Miwa
Division Of Biotechnology And Macromolecular Chemistry Graduate School Of Engineering Hokkaido Unive
-
Wakuda Akiko
Division Of Biotechnology And Macromolecular Chemistry Graduate School Of Engineering Hokkaido Unive
関連論文
- Dual production of poly(3-hydroxybutyrate) and glutamate using variable biotin concentrations in Corynebacterium glutamicum
- Improvement of Poly(3-Hydroxybutyrate)[P(3HB)] Production in Corynebacterium glutamicum by Codon Optimization, Point Mutation and Gene Dosage of P(3HB) Biosynthetic Genes(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
- Kinetic Analysis of Engineered Polyhydroxyalkanoate Synthases with Broad Substrate Specificity
- Production System for Biodegradable Polyester Polyhydroxybutyrate by Corynebacterium glutamicum
- Production of P(3-hydroxybutyrate-co-3-hydroxyhexanoate-co-3-hydroxyoctanoate) Terpolymers Using a Chimeric PHA Synthase in Recombinant Ralstonia eutropha and Pseudomonas putida
- The Hydrophobicity in a Chemically Modified Side-Chain of Cysteine Residues of Thanatin Is Related to Antimicrobial Activity against Micrococcus luteus
- Comparative Enzymatic Analysis of Azoreductases from Bacillus sp. B29
- Improved polyhydroxybutyrate (PHB) production in transgenic tobacco by enhancing translation efficiency of bacterial PHB biosynthetic genes(PLANT BIOTECHNOLOGY)
- A New Pathway for Poly(3-hydroxybutyrate) Production in Escherichia coli and Corynebacterium glutamicum by Functional Expression of a New Acetoacetyl-coenzyme A Synthase
- A New Beneficial Mutation in Pseudomonas sp. 61-3 Polyhydroxyalkanoate (PHA) Synthase for Enhanced Cellular Content of 3-Hydroxybutyrate-Based PHA Explored Using Its Enzyme Homolog as a Mutation Template
- Morphological Change in Cellular Granule Formation of Poly[(R)-3-hydroxybutyrate] Caused by DNA-Binding-Related Mutations of an Autoregulated Repressor PhaR
- Polyhydroxyalkanoates production from cellulose hydrolysate in Escherichia coli LS5218 with superior resistance to 5-hydroxymethylfurfural
- Development and validation of an HPLC-based screening method to acquire polyhydroxyalkanoate synthase mutants with altered substrate specificity(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
- Single-step production of polyhydroxybutyrate from starch by using α-amylase cell-surface displaying system of Corynebacterium glutamicum(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
- Single-step production of polyhydroxybutyrate from starch by using α-amylase cell-surface displaying system of Corynebacterium glutamicum