Morphological Change in Cellular Granule Formation of Poly[(R)-3-hydroxybutyrate] Caused by DNA-Binding-Related Mutations of an Autoregulated Repressor PhaR
スポンサーリンク
概要
- 論文の詳細を見る
PhaP is a major poly[(R)-3-hydroxybutyrate] [P(3HB)]-granule-associated protein. Its gene expression is controlled by an autoregulated repressor, PhaR, in Paracoccus denitrificans. The packing force of the P(3HB) granule by PhaP is greatly influenced by the number of PhaP molecules. In this study, the effects of DNA-binding-ability-reduced mutations of PhaR on morphological change in the cellular granule formation of P(3HB) were examined under a transmission electron microscope using an Escherichia coli recombinant system. Microscopic observation indicated that stronger packing of P(3HB) granules took place when the number of PhaP molecules was increased by reduction in the DNA-binding ability of PhaR.
- 社団法人 日本農芸化学会の論文
著者
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YAMADA Miwa
Division of Biotechnology and Macromolecular Chemistry, Graduate School of Engineering, Hokkaido Uni
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TAGUCHI Seiichi
Division of Biotechnology and Macromolecular Chemistry, Graduate School of Engineering, Hokkaido Uni
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WAKUDA Akiko
Division of Biotechnology and Macromolecular Chemistry, Graduate School of Engineering, Hokkaido Uni
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