Cloning, Expression and Purification of Bacillus cereus Endochitinase in the Escherichia coli AD494(DE3)pLysS Expression System
スポンサーリンク
概要
- 論文の詳細を見る
A chitinase gene from Bacillus cereus was cloned and expressed in Escherichia coli. The purified recombinant chitinase had much higher (128 fold) specificity to pNP-β-(GlcNAc)3 than to pNP-β-(GlcNAc), suggesting endochitinase. Thirty-three amino acids in the N-terminal were recognized and cut off during expression, which consequently made the Mr not correspond to that predicted.
- 社団法人 日本農芸化学会の論文
- 2009-05-23
著者
-
Jiang Shann-tzong
Department Of Food Science National Taiwan Ocean University
-
Chen Wei-ming
Department Of Electronics And Information Science Kyoto Institute Of Technology
-
Chen Wei-ming
Department Of Food Science National Taiwan Ocean University
-
Chen Ching-san
Institute Of Plant And Microbial Biology Academia Sinica
-
Chen Ching-san
Institute Of Botany Academia Sinica
-
CHEN Gen-Hung
Department of Cosmetic Science, Providence University
-
Chen Gen-hung
Department Of Cosmetic Science Providence University
関連論文
- Effect of met-myoglobin reductase on the color stability of blue fin tuna during refrigerated storage
- Purification and characterization of metmyoglobin reductase from ordinary muscle of blue-fin tuna
- Effect of polyethylene package on the metmyoglobin reductase activity and color of tuna muscle during low temperature storage
- Purification and characterization of three amylases from viscera of hard clam Meretrix lusoria
- Enhancement of the expression of ice-nucleation activity of Pseudomonas fluorescens MACK-4 isolated from mackerel
- Effects of mackerel cathepsins L and L-like, and calpain on the degradation of mackerel surimi
- Effects of Eution Conditions on the Separation of Calpastatin, μ-and m-Calpain on DEAE-Sephacel Chromatography
- Properties of extracellular ice-nucleating substances from Pseudomonas fluorescens MACK-4 and its effect on the freezing of some food materials
- Monte-Carlo Simulation of Surface Reactions in Plasma-Enhanced Chemical Vapor Deposition of Hydrogenated Amorphous Silicon Thin Films
- Cloning, Expression and Purification of Bacillus cereus Endochitinase in the Escherichia coli AD494(DE3)pLysS Expression System
- Purification and Characterization of β-D-Glucosidases from Euphausia superba(Biological Chemistry)