Preparation and Characterization of Branched β-Cyclodextrins Having α-_L-Fucopyranose and a Study of Their Functions
スポンサーリンク
概要
- 論文の詳細を見る
Three positional isomers of 61,6n-di-O-(α-L-fucopyranosyl)-β-cyclodextrin [61,6n-di-O-(α-L-Fuc)-βCD, n=2–4] were chemically synthesized by using the corresponding authentic compounds, 61,6n-di-O-(tert-butyldimethylsilyl)-βCD (n=2–4) as fucosyl acceptors and 2,3,4-tri-O-benzyl-L-fucopyranosyl trichloroacetimidate as a fucosyl donor. Their structures were analyzed by HPLC, MS and NMR spectroscopy. The hemolytic activities of the α-L-Fuc-βCDs were lower than that of βCD, while the water solubility of these branched βCDs was much higher than that of βCD. The molecular interaction between these compounds and the fucose-binding lectin, Aleuria aurantia lectin (AAL), was investigated by using an optical biosensor based on the surface plasmon resonance (SPR) technique. The order of binding affinity, as a function of the fucose-binding position, was 61,64- > 61,63- > 61,62-di-O-(α-L-Fuc)-βCD > 6-O-(α-L-Fuc)-βCD.
- 社団法人 日本農芸化学会の論文
- 2009-03-23
著者
-
Tanimoto Toshiko
School Of Pharmaceutical Science Mukogawa Women's University
-
Nishi Yuki
School Of Pharmaceutical Sciences Mukogawa Women's Univ.
関連論文
- Microdose clinical trial : Quantitative determination of fexofenadine in human plasma using liquid chromatography/electrospray ionization tandem mass spectrometry
- Enzymatic Synthesis of N-Acetylglucosaminyl-cyclodextrin by the Reverse Reaction of N-Acetylhexosaminidase from Jack Bean
- Synthesis of Novel Heterobranched β-Cyclodextrins Having β-D-N-Acetylglucosaminyl-Maltotriose on the Side Chain
- Preparation and Characterization of Novel Branched β-Cyclodextrins Having β-D-Galactose Residues on the Non-reducing Terminal of the Side Chains and Their Specific Interactions with Peanut (Arachis hypogaed) Agglutinin
- Synthesis of Novel Heterobranched β-Cyclodextrins from α-D-Mannosyl Maltotriose and β-Cyclodextrin by the Reverse Action of Pullulanase, and Isolation and Characterization of the Products
- Synthesis of Novel Heterobranched β-Cyclodextrins from 4^2-O-β-D-Galactosyl-Maltose and β-Cyclodextrin by the Reverse Action of Pullulanase, and Isolation and Characterization of the Products
- Enzymatic Synthesis of Mannosyl-cyclodextrin by α-Mannosidase from Jack Bean
- HPLC Analysis of Manno-Oligosaccharides Derived from Saccharomyces cerevisiae Mannan Using an Amino Column or a Graphitized Carbon Column
- Determination of Branched β-Cyclodextrin-Prostaglandin Complexes Using Electrospray Ionization Mass Spectrometry
- Preparation and Characterization of Branched β-Cyclodextrins Having α-_L-Fucopyranose and a Study of Their Functions
- 緒言 : 糖質科学と糖質工学の産業的利用