A Maltooligosaccharide-Forming Amylase Gene from Brachybacterium sp. Strain LB25 : Cloning and Expression in Escherichia coli
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概要
- 論文の詳細を見る
Brachybacterium sp. strain LB25 produces a maltooligosaccharide-forming amylase that improves product selectivity in water-miscible organic solvents. The enzyme hydrolyzed starch to produce maltotriose primarily. The structural gene encoding the amylase from strain LB25 was cloned and sequenced. The amino acid sequence of the product showed significant similarity (45 to 49%) to amylases from the genus Streptomyces. The amylase gene was expressed in Escherichia coli, but the specific activity of the recombinant amylase was lower than that of the amylase purified from strain LB25.
- 社団法人 日本農芸化学会の論文
- 2008-09-23
著者
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Ogino Hiroyasu
Department Of Bioengineering Faculty Of Bioscience And Biotechnology Tokyo Institute Of Technology
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Ogino Hiroyasu
Department Of Chemical Engineering Osaka Prefecture University
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Doukyu Noriyuki
Bio-nano Electronic Research Center Toyo University
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YAMAGISHI Wataru
Department of Life Science, Toyo University
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KUWAHARA Hirokazu
Extremobiosphere Research Center, Japan Agency for Marine-Earth Science and Technology (JAMSTEC)
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Yamagishi Wataru
Department Of Life Science Toyo University
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Kuwahara Hirokazu
Extremobiosphere Research Center Japan Agency For Marine-earth Science And Technology (jamstec)
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