Protection of psbAII Transcript from Ribonuclease Degradation in Vitro by DnaK2 and DnaJ2 Chaperones of the Cyanobacterium Synechococcus elongatus PCC 7942
スポンサーリンク
概要
- 論文の詳細を見る
Three dnaK and four dnaJ genes have been identified in the genome of cyanobacterium Synechococcus elongatus PCC 7942. Our comprehensive analysis of yeast two-hybrid screening revealed a specific interaction among DnaK2, DnaJ2, and RNase E, an essential endoribonuclease. We examined the effects of DnaK2 and DnaJ2 on RNase E activity by monitoring the digestion of psbAII transcript in vitro. The addition of DnaK2 and DnaJ2 obviously inhibited RNase E activity in an ATP-dependent manner. These results suggest that DnaK2 and DnaJ2 are involved in RNA degradation through interaction with RNase E.
- 社団法人 日本農芸化学会の論文
- 2007-01-23
著者
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WATANABE Satoru
Department of Biocybernetics, Niigata University
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Yoshikawa Hirofumi
Department of Bioscience, Tokyo University of Agriculture
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Sato Masumi
Department Of Bioscience Tokyo University Of Agriculture
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NIMURA MATSUNE
Department of Bioscience, Tokyo University of Agriculture
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CHIBAZAKURA Taku
Department of Bioscience, Tokyo University of Agriculture
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Nimura Matsune
Department Of Bioscience Tokyo University Of Agriculture
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Yoshikawa Hirofumi
Dep. Of Bioscience Tokyo Univ. Of Agriculture
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Yoshikawa Hirofumi
Department Of Bioscience Tokyo University Of Agriculture
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Yoshikawa Hirofumi
Department Of Basic Science Graduate School Of Arts And Sciences The University Of Tokyo
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Watanabe Satoru
Department Of Astronomy School Of Science The University Of Tokyo
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Chibazakura Taku
Dep. Of Bioscience Tokyo Univ. Of Agriculture
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Chibazakura Taku
Department Of Bioscience Tokyo University Of Agriculture
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Watanabe Satoru
Department of Bioscience, Tokyo University of Agriculture
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Nimura-Matsune Kaori
Department of Bioscience, Tokyo University of Agriculture
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