Salmonella Typhimurium Isolated from Healthy Pigs and Their Ability of Horizontal Transfer of Multidrug Resistance and Virulence Genes
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概要
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Salmonella is the most common food-borne pathogen worldwide. Rapid dissemination of multidrug-resistant strains, in particular from animal origins, is a growing concern to human and animal health, and it is mostly attributed to conjugative DNA exchange in the intestinal tract of food animals. To understand the potential role of pigs as a reservoir for antimicrobial-resistant Salmonella, we isolated Salmonella from slaughtered pigs and examined their drug resistance and gene transfer ability. We collected fecal and carcass swabs from 104 healthy pigs at an abattoir in Obihiro, Hokkaido, Japan. A total of 15 Salmonella strains were isolated, the most common serotypes being S. Typhimurium (7/15), S. Derby (2/15), S. Southampton (2/15) and S. O4:d:- (4/15). All of S. Typhimurium isolates were resistant to at least one of five antibiotics (ampicillin, kanamycin, sulfisoxazole, tetracycline and streptomycin). Pulsed-field gel electrophoresis (PFGE) profiles after XbaI and BlnI digestion were analyzed. S. Typhimurium isolates from 3 farms located in different regions clustered together and showed genetic relatedness. In conjugation experiments, one multidrug-resistant S. Typhimurium isolate showed the ability to transfer not only antibiotic resistance genes but also virulence genes such as spvABC to recipient bacteria. These results suggest that the spread of S. Typhimurium had occured in pig farms and that asymptomatic Salmonella-infected pigs should be considered as a significant source of antibiotic-resistant bacteria.
- 2009-10-01
著者
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TAKESHI Koichi
Department of Applied Veterinary Science and Public Health, Obihiro University of Agriculture and Ve
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MAKINO Sou-Ichi
Laboratory of Food Microbiology and Immunology, Research Center for Animal Hygiene and Food Safety,
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Thuy Nguyen
Obihiro Univ. Hokkaido
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Thuy Nguyen
Laboratory of Food Microbiology and Immunology, Research Center for Animal Hygiene and Food Safety,
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Kusumoto Akiko
Laboratory of Food Microbiology and Immunology, Research Center for Animal Hygiene and Food Safety,
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Kawamoto Keiko
Laboratory of Food Microbiology and Immunology, Research Center for Animal Hygiene and Food Safety,
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Takeshi Koichi
帯広畜産大学 動物医科学分野
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Takeshi Koichi
Department Of Applied Veterinary Science And Public Health Obihiro University Of Agriculture And Vet
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Kawamoto Keiko
Laboratory Of Food Microbiology & Immunology Research Center For Animal Hygiene And Food Safety
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Makino Sou-ichi
Laboratory Of Food Microbiology And Immunology Research Center For Animal Hygiene And Food Safety Ob
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Makino Sou-ichi
Department Of Veterinary Microbiology University Of Agriculture And Veterinary Medicine
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Makino Sou-ichi
Departments Of Veterinary Microbiology Obihiro University Of Agriculture And Veterinary Medicine
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Makino Sou-ichi
Laboratory Of Food Microbiology & Immunology Research Center For Animal Hygiene And Food Safety
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Kusumoto Akiko
Laboratory Of Food Microbiology And Immunology Research Center For Animal Hygiene And Food Safety Ob
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Takeshi Koichi
Department Of Applied Veterinary Medicine Obihiro University Of Agriculture And Veterinary Medicine
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Kawamoto Keiko
Laboratory Of Food Microbiology And Immunology Research Center For Animal Hygiene And Food Safety Ob
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Thuy Nguyen
Laboratory Of Food Microbiology And Immunology Research Center For Animal Hygiene And Food Safety Ob
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