Epsilon-Polylysine Microparticle Adjuvant Drives Cytokine Production to Th1 Profile

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概要

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• Epsilon-polylysine micro particles (SGEPL) and polyethyleneimine micro particles (SGPEI) were developed by the addition of a hydrophobic group and the immunological characterization of these micro particles and aluminum hydroxide (ALUM) was investigated. BALB/c mice were injected intraperitoneally with ovalbumin (OVA) as an antigen and SGEPL, SGPEI or ALUM as an adjuvant. The results showed that the mice injected with SGEPL produced a significant portion of anti-OVA antibody subclass IgG2a in the sera and suppressed interleukin (IL)-4 and IL-5, but enhanced IL-12 and interferon-γ (IFN-γ) from the spleen cells. Similar results relating to cytokines were also obtained, even without OVA. Direct stimulation with SGEPL to naïve BALB/c mouse spleen cells induced IL-12 and IFN-γ. Both spleen and purified B cells produced IgG1 and IgE after stimulation with IL-4 and the anti-CD40 monoclonal antibody. With the addition of SGEPL, the IgE production from the cells was suppressed as a result of enhanced IFN-γ production. Furthermore, IgE production was also suppressed in the purified B cells without the influence of IFN-γ or IL-12. Thus, we suggest SGEPL drives cytokine production to Th1 profile. It will be a novel promising adjuvant based on this viewpoint.

著者

• Kawamoto Keiko

  Laboratory Of Food Microbiology & Immunology Research Center For Animal Hygiene And Food Safety

• Makino Sou-ichi

  Laboratory Of Food Microbiology & Immunology Research Center For Animal Hygiene And Food Safety

• Matsuda Hiroshi

  Laboratory Of Clinical Immunology Department Of Veterinary Medicine Faculty Of Agriculture Tokyo University Of Agriculture And Technology

• Kawamoto Keiko

  Laboratory of Food Microbiology and Immunology, Research Center for Animal Hygiene and Food Safety, Obihiro University of Agriculture and Veterinary Medicine

• WAKAMOTO Hiroaki

  Basic Veterinary Science, Doctoral Course of the United Graduate School of Veterinary Sciences, Gifu University

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