ドジョウ (Misgurnus anguillicaudatus) のゲノムにおける異なったフォームのトランスジーンの導入効率の評価
スポンサーリンク
概要
- 論文の詳細を見る
To assess the effects of different transgene forms on integration efficiency, plasmids containing human growth hormone gene under the control of the metallothionein promoter (pMThGH) in circular, linearized forms and lyposome trapped linearized plasmid were introduced into fertilized loach eggs. Prior to microinjection, plasmids were identified for the presence of human growth hormone (hGH) gene by digestion with restriction enzymes (EcoRI, BamHI, BglII) and by polymerase chain reaction (PCR). The linearized form of pMThGH was generated by digesting circular plasmids with BamHI. Liposome trapped linearized plasmid was prepared in the presence of dioleyl phosphatidyl choline, cholesterol and pMThGH. The presence of hGH genes in the genomic DNA of loach developed from microinjected eggs was identified by a PCR method using two hGH-specific primers. PCR products of the positive individuals showed one band corresponding to 479 bp, while those of negative and control individuals showed no corresponding band. Integration rates of hGH genes into fish chromosomes were 6%, 12% and 11% for circular, linearized plasmids and lyposome trapped linearized plasmid, respectively. In accordance with above order, the average body weight of transgenic loach at 6 months was increased by 1.63, 1.73 and 1.68 times over controls, respectively. The results indicate that plasmids in linearized form and lyposome trapped linearized plasmid were more efficiently integrated into loach chromosomes than circular ones, but there was little significant difference in body weight among the 3 groups of transgenic fish.
- 日本繁殖生物学会の論文
- 2002-04-01
著者
-
Thuy N
Institute Of Biotechnology National Center For Natural Science & Technology
-
Van Cuong
Institute Of Biotechnology National Center For Natural Science & Technology
-
Thuy Nguyen
Institute Of Biotechnology National Center For Natural Science & Technology
-
THI Quyen
Institute of Biotechnology, National Center for Natural Science & Technology
-
ANH Dau
Institute of Biotechnology, National Center for Natural Science & Technology
-
NGA Tham
Institute of Biotechnology, National Center for Natural Science & Technology
-
Nga Tham
Institute Of Biotechnology National Center For Natural Science & Technology
-
Anh Dau
Institute Of Biotechnology National Center For Natural Science & Technology
-
Thi Quyen
Institute Of Biotechnology National Center For Natural Science & Technology
-
Thuy Nguyen
Institute Of Archeology
-
Cuong Nguyen
Institute Of Archeology
関連論文
- New Triterpenoid Saponins from Glochidion eriocarpum and Their Cytotoxic Activity
- Chemical Constituents from the Leaves of Manglietia phuthoensis and Their Effects on Osteoblastic MC3T3-E1 Cells
- New Cembranoid Diterpenes from the Vietnamese Soft Coral Sarcophyton mililatensis Stimulate Osteoblastic Differentiation in MC3T3-E1 Cells
- A New Monoterpenoid Glycoside from Myrica esculenta and the Inhibition of Angiotensin I-Converting Enzyme
- α-Glucosidase Inhibition Properties of Cucurbitane-Type Triterpene Glycosides from the Fruits of Momordica charantia
- ドジョウ (Misgurnus anguillicaudatus) のゲノムにおける異なったフォームのトランスジーンの導入効率の評価
- A New Dimeric Carbazole Alkaloid from Glycosmis stenocarpa Roots
- Morphometric affinity of the late Neolithic human remains from Man Bac, Ninh Binh Province, Vietnam: key skeletons with which to debate the ‘two layer’ hypothesis
- Terminal Pleistocene human skeleton from Hang Cho Cave, northern Vietnam: implications for the biological affinities of Hoabinhian people
- New Pyrano-Pyrone from Goniothalamus tamirensis Enhances the Proliferation and Differentiation of Osteoblastic MC3T3-E1 Cells
- On the least degree of polynomials bounding above the differences between lengths and multiplicities of certain systems of parameters in local rings
- On the length of the powers of systems of parameters in local ring
- Total Peroxynitrite Scavenging Capacity of Phenylethanoid and Flavonoid Glycosides from the Flowers of Buddleja officinalis
- A characterization for pseudo Buchsbaum modules
- Preparation and Antitumor-promoting Activity of Curcumin Encapsulated by 1,3-β-Glucan Isolated from Vietnam Medicinal Mushroom Hericium erinaceum
- Oleanolic Triterpene Saponins from the Roots of Panax bipinnatifidus
- Terminal Pleistocene human skeleton from Hang Cho Cave, northern Vietnam : implications for the biological affinities of Hoabinhian people
- Morphometric affinity of the late Neolithic human remains from Man Bac, Ninh Binh Province, Vietnam : key skeletons with which to debate the 'two layer' hypothesis
- Labdane-Type Diterpenoids from the Rhizomes of Hedychium coronarium Inhibit Lipopolysaccharide-Stimulated Production of Pro-inflammatory Cytokines in Bone Marrow-Derived Dendritic Cells
- Diterpenoids from the Soft Coral Sinularia maxima and Their Inhibitory Effects on Lipopolysaccharide-Stimulated Production of Pro-inflammatory Cytokines in Bone Marrow-Derived Dendritic Cells