Non-thermal cytocidal effect of infrared irradiation on cultured cancer cells using specialized device
スポンサーリンク
概要
- 論文の詳細を見る
The definitive version is available at www.blackwell-synergy.comAs infrared penetrates the skin, thermal effects of infrared irradiation on cancer cells have been investigated in the field of hyperthermia. We evaluated non-thermal effects of infrared irradiation using a specialized device (1100-18000 nm with filtering of wavelengths between 1400 and 1500 nm and contact cooling) on cancer cells. In in vitro study, five kinds of cultured cancer cell lines (MCF7 breast cancer, HeLa uterine cervical cancer, NUGC-4 gastric cancer, B16F0 melanoma, and MDA-MB435 melanoma) were irradiated using the infrared device, and then the cell proliferation activity was evaluated by 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay. Proliferation of all the cancer cell lines was significantly suppressed by infrared irradiation. Total infrared output appeared to be correlated with cell survival. Increased temperature during infrared irradiation appeared not to play a role in cell survival. The maximum temperature elevation in the wells after each shot in the 20 and 40 J/cm2 culture was 3.8 degrees C and 6.9 degrees C, respectively. In addition, we have shown that infrared irradiation significantly inhibited the tumor growth of MCF7 breast cancer transplanted in severe combined immunodeficiency mice and MDA-MB435 melanoma transplanted in nude mice in vivo. Significant differences between control and irradiated groups were observed in tumor volume and frequencies of TUNEL-positive and Ki-67-positive cells. These results indicate that infrared, independent of thermal energy, can induce cell killing of cancer cells. As this infrared irradiation schedule reduces discomfort and side effects, reaches the deep subcutaneous tissues, and facilitates repeated irradiations, it may have potential as an application for treating various forms of cancer.
- 2010-06-10
著者
-
Nakayama Jun
Department of Clinical Medicine, Shinshu University, School of Medicine
-
TANAKA Yohei
Department of Mechanical and Aerospace Engineering, Tokyo Institute of Technology
-
MATSUO Kiyoshi
Department of Plastic and Reconstructive Surgery, Shinshu University Graduate School of Medicine
-
YUZURIHA Shunsuke
Department of Plastic and Reconstructive Surgery, Shinshu University Graduate School of Medicine
-
YAN Huimin
Department of Molecular Pathology, Shinshu University Graduate School of Medicine
-
Tanaka Yohei
Department Of Plastic And Reconstructive Surgery Shinshu University Graduate School Of Medicine
-
Yan Huimin
Department Of Molecular Pathology Shinshu University Graduate School Of Medicine
-
Matsuo Kiyoshi
Department Of Plastic And Reconstructive Surgery Shinshu University Graduate School Of Medicine
-
Yuzuriha Shunsuke
Department Of Plastic And Reconstructive Surgery Shinshu University Graduate School Of Medicine
-
Nakayama Jun
Department Of Molecular Pathology Shinshu University Graduate School Of Medicine
-
Nakayama Jun
Department Of Clinical Medicine Shinshu University School Of Medicine
関連論文
- Flight Model Development of Tokyo Tech Nano-Satellite Cute-1.7 + APD II
- Tumor Necrosis Factor (TNF) とInterleukin (IL)-1の直接注入による脳病変
- Non-thermal cytocidal effect of infrared irradiation on cultured cancer cells using specialized device
- Non-thermal cytocidal effect of infrared irradiation on cultured cancer cells using specialized device
- A Rare Lung Nodule Consisting of Adenocarcinoma and Amyloid Deposition in a Patient with Primary Systemic AL Amyloidosis
- S1-3 Role of gland mucous cell-type mucin in the gastric mucosa infected with Helicobacter pylori(THE 45TH ANNUAL MEETING OF THE JAPAN SOCIETY OF HISTOCHEMISTRY AND CYTOCHEMISTRY)
- CT and MR Findings of a Pleomorphic Adenoma in the Peripheral Lung
- Ontogeny of the Rat Parietal Cell: Analysis Using Anti- parietal Cell Antibody and Transmission Electron Microscopy
- P-812 Enhanced Expression of Core 2 β -1,6-N-acetylglucosaminyltransferase (C2GnT) Gene in Human Lung Cancer
- Correlation between glial fibrillary acidic protein-positive astrocytes and age in the human hippocampus
- II-B-28 Increased expression of a vanilloid receptor, TRPV1 in rat stellate ganglia induced by inflammatory stimuli and its down regulation by RNA interference(THE 45TH ANNUAL MEETING OF THE JAPAN SOCIETY OF HISTOCHEMISTRY AND CYTOCHEMISTRY)
- P-2 Effect of exhaustive exercise on TLR4 expression in macrophages in mice(Free communication (Poster),8^ INTERNATIONAL SOCIETY OF EXERCISE AND IMMUNOLOGY SYMPOSIUM,ISEI2007 INFLAMMATION IN EXERCISE FRIEND OR FOE?)
- Tranilast Suppresses the Disease Development of the Adjuvant-and Streptococcal Cell Wall-Induced Arthritis in Rats
- Female adnexal tumor of probable wolffian origin : Morphological, immunohistochemical, and ultrastructural study with c-kit gene analysis
- Pulmonary Lymphoepithelioma-like Carcinoma : Predominant Infiltration of Tumor-associated Cytotoxic T Lymphocytes Might Represent the Enhanced Tumor Immunity
- Clinical utility of quantitative RT-PCR targeted to α1,4-N-acetylglucosaminyltransferase mRNA for detection of pancreatic cancer
- Identification of oligopeptides binding to peritoneal tumors of gastric cancer
- Liver Organogenesis from Murine Embryonic Stem Cells(International Symposium of Maxillofacial and Oral Regenerative Biology in Okayama 2005)
- Application of Mucin Histochemistry for Pathological Diagnosis : Expression of Gastric Phenotypes in Metaplastic and Neoplastic Lesions and its Relation to the Organoid Differentiation
- Histo- and Cytochemistry of Gland Mucus Cell Type Mucin and Its Practical Application for Pathologic and Clinical Diagnosis
- Malignant myoepithelioma (myoepithelial carcinoma) of soft tissue
- The Utility of Formalin-fixed and Paraffin-embedded Tissue Blocks for Quantitative Analysis of N-acetylgalactosamine 4-sulfate 6-O-sulfotransferase mRNA Expressed by Colorectal Cancer Cells
- A Case of Ganglioneuroma with Fatty Replacement:CT and MRI Findings
- Role of Sulfated O-Glycans Expressed by High Endothelial Venule-Like Vessels in Pathogenesis of Chronic Inflammatory Gastrointestinal Diseases
- Therapeutic and Preventive Effects of Methotrexate on Zymosan-Induced Arthritis in SKG Mice
- Arachidonic Acid-Induced COX-1 and COX-2-Mediated Vasodilation in Rat Gingival Arterioles In Vivo
- IIB-10 Lymphangiogenesis occurred in colorectal carcinoma and its clinicopathological significance(THE 46TH ANNUAL MEETING OF THE JAPAN SOCIETY OF HISTOCHEMISTRY AND CYTOCHEMISTRY)
- S3-02 Application of immunohistochemistry for study of ASC-related molecules in diseases(Advanced Histochemistry)
- Immunohistochemical demonstration of apoptosis-regulated proteins, Bcl-2 and Bax, in resected non-small-cell lung cancers
- Spiral Cleavage of Mica
- Pulmonary Tumor Thrombotic Microangiopathy Diagnosed Antemortem and Treated with Combination Chemotherapy
- Primary Malignant Sarcomatoid Mesothelioma in the Pericardium