LAMP法 (Loop-Mediated Isothermal Amplificatopn) を用いたクリプトスポリジウムおよびジアルジアの高感度迅速検出

概要

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To establish a new method of detecting Cryptosporidium and Giardia in source and drinking water, we designed and evaluated a loop-mediated isothermal amplification (LAMP) assay, using a set of primers targeting of the 18S rRNA genes of Cryptosporidium and Giardia. The method showed a rapid and specific amplification of all the strains of Cryptosporidium and Giardia tested within 1 hour at 63°C. The detection limit of either protozoon was 0.8 (oo)cysts/test. The LAMP assay was considered to be used for testing the presence of Cryptosporidium and/or Giardia in drinking water having diagnostic high sensitivity and specificity.
2009-06-10