Searching for genetic factors involved in hybrid lethality in Nicotiana ; Possible use of a cultured cell line of a hybrid
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概要
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The cell line GTH4 generated from the F1 hybrid of Nicotiana gossei×N. tabacum reveals lethality at the seedling stage. The cells proliferated at 37°C but died at 26°C. GTH4S, a cell line derived from GTH4 that does not die at 26°C, was established. Differences in the genes and proteins expressed in the two cell lines were analyzed. GTH4 expressed the gene encoding ubiquitin-conjugating enzyme (E2) which was not expressed in GTH4S. PCR targeting this sequence indicated that GTH4S is missing a segment of DNA containing the E2 locus, suggesting that GTH4S is a deletion mutant of GTH4. An analysis of proteins using anion-exchange HPLC and SDS-PAGE revealed GTH4 to contain protein species not found in GTH4S. The genes and/or proteins involved in the expression of hybrid lethality can be analyzed by comparing these two cell lines.
- 2006-12-01
著者
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Sato Kenji
Department of Anatomy and Physiological Science, Graduate School of Health Care Sciences, Tokyo Medi
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YAMAGUCHI Yuki
Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology
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Mino Masanobu
Graduate School Of Agriculture Kyoto Prefectural University
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KUBOTA Mami
Graduate School of Agriculture, Kyoto Prefectural University
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KAWAMORI Tetsu
Graduate School of Agriculture, Kyoto Prefectural University
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MISAKA Yuko
Graduate School of Agriculture, Kyoto Prefectural University
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TANAKA Yoshikazu
The Wakasawan Energy Research Center
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INOUE Masayoshi
Graduate School of Agriculture, Kyoto Prefectural University
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Yamaguchi Yuki
Graduate School Of Agriculture Kyoto Prefectural University
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Misaka Yuko
Graduate School Of Agriculture Kyoto Prefectural University
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Kubota Mami
Graduate School Of Agriculture Kyoto Prefectural University
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Kawamori Tetsu
Graduate School Of Agriculture Kyoto Prefectural University
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Sato Kenji
Department Of Anatomy And Physiological Science Graduate School Of Health Care Sciences Tokyo Medica
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Inoue Masayoshi
Graduate School Of Agriculture Kyoto Prefectural University
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Sato Kenji
Department Of Food Science And Nutritional Health Kyoto Prefectural University
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