In vitro Evaluation of Small RNA Accumulation in Chrysanthemum Mediated by Chrysanthemum Stunt Viroid

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概要

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• A comparative analysis of stunting was conducted at various temperatures to develop a simple and reliable method for evaluating the disease symptoms of the chrysanthemum stunt viroid (CSVd). CSVd-free plants had significantly greater plant height than CSVd-carrying plants at 22°C and 24°C. No significant differences in plant height were detected at high and low temperatures. These results suggest that the in vitro evaluation of plant growth at an appropriate temperature might provide a useful method for CSVd diagnosis. In addition, the intensity of the small RNA hybridization signal obtained by northern blot analysis using a full-length CSVd RNA as a probe indicated synchronous behavior that correlated with the degree of growth suppression. These results suggest that a small RNA from CSVd may lead directly to the suppression of plant growth, with a similar function to siRNAs in chrysanthemum.

著者

• Mino Masanobu

  Graduate School Of Agriculture Kyoto Prefectural University

• Tsuro Masato

  Faculty Of Agriculture Meijo University

• MINO Masanobu

  Graduate School of Environmental Life Science, Kyoto Prefectural University

• SAKUMA Atsuko

  Faculty of Agriculture, Meijo University

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