Molecular-based method to distinguish sympatric deep-sea whelks, Buccinum striatissimum and Buccinum tenuissimum in the Sea of Japan
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概要
- 論文の詳細を見る
Buccinum striatissimum and Buccinum tenuissimum are commercially important whelks in the Sea of Japan. B. striatissimum is very similar to B. tenuissimum and their morphological variations make the identification of these species difficult, especially in case that these species are caught together because they are subject to the same environmental factors. In order to develop an easy and objective method to delineate these species, we utilized the difference of mitochondrial 16SrDNA of these two species. As a result, we could easily distinguish these two species by PCR-RFLP method using the difference of this region and AseI restriction endonuclease. In addition, we succeeded in distinguishing sympatric samples of these species, which suggests that these two species are likely to be reproductively isolated although the possibility of hybridization can not be completely ruled out.
- 日本プランクトン学会、日本ベントス学会の論文
- 2008-05-01
著者
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TOYOHARA Haruhiko
Division of Applied Biosciences, Graduate School of Agriculture, Kyoto University
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Hayashi Isao
Division Of Applied Biosciences Graduate School Of Agriculture Kyoto University
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IGUCHI AKIRA
Division of Applied Biosciences, Graduate School of Agriculture, Kyoto University
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UENO MASAHIRO
Field Science Education and Research Center, Kyoto University
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MAEDA TSUNEO
Toyama Prefecture Fisheries Research Institute
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MINAMI TAKASHI
Japan Sea National Fisheries Research Institute
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Iguchi Akira
Division Of Applied Biosciences Graduate School Of Agriculture Kyoto University
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Ueno M
Field Science Education And Research Center Kyoto University
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Ueno Masahiro
Field Science Education And Research Center Kyoto University
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Toyohara Haruhiko
Division Of Applied Biosciences Graduate School Of Agriculture Kyoto University
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Toyohara Haruhiko
Laboratory Of Fishery Chemistry Department Of Fisheries Faculty Of Agriculture Kyoto University
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Hayashi Ikuo
Division Of Applied Biosciences Graduate School Of Agriculture Kyoto University
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Maeda Tatsuaki
Toyama Prefecture Fisheries Research Institute
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Toyohara H
Division Of Applied Biosciences Graduate School Of Agriculture Kyoto University
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Hayashi Isao
Division Of Applied Bioscience Graduate School Of Agriculture Kyoto University
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- Molecular-based method to distinguish sympatric deep-sea whelks, Buccinum striatissimum and Buccinum tenuissimum in the Sea of Japan
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