Isolation and Structure Determination of Bioactive Isoflavones from Callus Culture of Dipteryx odorata

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概要

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• Crude extracts of callus and roots of Dipteryx odorata were analyzed by HPLC to detect and quantify isoflavone contents. Based on spectroscopic and X-ray crystallography data the structures of two isoflavones were elucidated as 7-hydroxy-4', 6-dimethoxyisoflavone and 3', 7-dihydroxy-4', 6-dimethoxyisoflavone. The production of dry biomass of 7-hydroxy-4', 6-dimethoxyisoflavone in cultured callus was 4.12mg/g, approximately eleven fold higher than the amount accumulated in roots of D. odorata wild-growing plants. The 7-hydroxy-4', 6-dimethoxyisoflavone was effective against glyceraldehyde-3-phosphate dehydrogenase from Trypanosoma cruzi. This is the first report on those bioactive isoflavones accumulated in callus of D. odorata.

• 公益社団法人日本薬学会の論文
• 2005-07-01

著者

• Vieira Paulo

  Departamento De Quimica Universidade Federal De Sao Carlos

• Fernandes Joao

  Departamento De Quimica Universidade Federal De Sao Carlos

• Vieira Paulo

  Departamento De Quimica Universidade Federal De Sao Carlos-ufscar

• Fernandes Joao

  Departamento De Quimica Universidade Federal De Sao Carlos-ufscar

• Castilho Marcelo

  Instituto De Fisica Universidade De Sao Paulo-usp

• Franca Suzelei

  Departamento De Biotecnologia Vegetal Universidade De Ribeirao Preto

• Franca Suzelei

  Departamento De Quimica Universidade Federal De Sao Carlos-ufscar

• Lourenco Miriam

  Unidade De Biotecnologia Universidade De Ribeirao Preto Unaerp

• Da Silva

  Departamento De Bioquimica Instituto De Quimica Universidade Federal Do Rio De Janeiro

• JANUARIO Ana

  Unidade de Biotecnologia, Universidade de Ribeirao Preto, UNAERP

• DOMEZIO Lilian

  Unidade de Biotecnologia, Universidade de Ribeirao Preto, UNAERP

• PIETRO Rosemeire

  Departamento de Ciencias Farmaceuticas, Universidade de Ribeirao Preto-UNAERP

• TOMAZELA Daniela

  Sao Carlos

• Januario Ana

  Unidade De Biotecnologia Universidade De Ribeirao Preto

• Januario Ana

  Unidade De Biotecnologia Universidade De Ribeirao Preto Unaerp

• Domezio Lilian

  Unidade De Biotecnologia Universidade De Ribeirao Preto Unaerp

• Pietro Rosemeire

  Departamento De Ciencias Farmaceuticas Universidade De Ribeirao Preto-unaerp

• Da Silva

  Departamento De Biologia Celular Universidade De Brasilia

• Da Silva

  Departamento De Quimica Universidade Federal De Sao Carlos-ufscar

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