Isolation and Structure Determination of Bioactive Isoflavones from Callus Culture of Dipteryx odorata
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概要
- 論文の詳細を見る
Crude extracts of callus and roots of Dipteryx odorata were analyzed by HPLC to detect and quantify isoflavone contents. Based on spectroscopic and X-ray crystallography data the structures of two isoflavones were elucidated as 7-hydroxy-4', 6-dimethoxyisoflavone and 3', 7-dihydroxy-4', 6-dimethoxyisoflavone. The production of dry biomass of 7-hydroxy-4', 6-dimethoxyisoflavone in cultured callus was 4.12mg/g, approximately eleven fold higher than the amount accumulated in roots of D. odorata wild-growing plants. The 7-hydroxy-4', 6-dimethoxyisoflavone was effective against glyceraldehyde-3-phosphate dehydrogenase from Trypanosoma cruzi. This is the first report on those bioactive isoflavones accumulated in callus of D. odorata.
- 公益社団法人日本薬学会の論文
- 2005-07-01
著者
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Vieira Paulo
Departamento De Quimica Universidade Federal De Sao Carlos
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Fernandes Joao
Departamento De Quimica Universidade Federal De Sao Carlos
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Vieira Paulo
Departamento De Quimica Universidade Federal De Sao Carlos-ufscar
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Fernandes Joao
Departamento De Quimica Universidade Federal De Sao Carlos-ufscar
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Castilho Marcelo
Instituto De Fisica Universidade De Sao Paulo-usp
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Franca Suzelei
Departamento De Biotecnologia Vegetal Universidade De Ribeirao Preto
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Franca Suzelei
Departamento De Quimica Universidade Federal De Sao Carlos-ufscar
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Lourenco Miriam
Unidade De Biotecnologia Universidade De Ribeirao Preto Unaerp
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Da Silva
Departamento De Bioquimica Instituto De Quimica Universidade Federal Do Rio De Janeiro
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JANUARIO Ana
Unidade de Biotecnologia, Universidade de Ribeirao Preto, UNAERP
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DOMEZIO Lilian
Unidade de Biotecnologia, Universidade de Ribeirao Preto, UNAERP
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PIETRO Rosemeire
Departamento de Ciencias Farmaceuticas, Universidade de Ribeirao Preto-UNAERP
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TOMAZELA Daniela
Sao Carlos
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Januario Ana
Unidade De Biotecnologia Universidade De Ribeirao Preto
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Januario Ana
Unidade De Biotecnologia Universidade De Ribeirao Preto Unaerp
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Domezio Lilian
Unidade De Biotecnologia Universidade De Ribeirao Preto Unaerp
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Pietro Rosemeire
Departamento De Ciencias Farmaceuticas Universidade De Ribeirao Preto-unaerp
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Da Silva
Departamento De Biologia Celular Universidade De Brasilia
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Da Silva
Departamento De Quimica Universidade Federal De Sao Carlos-ufscar
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