Characterization of Murine Grancalcin Specifically Expressed in Leukocytes and Its Possible Role in Host Defense against Bacterial Infection

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概要

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• Such phagocytic leukocytes as macrophages and neutrophils are the key cellular components of innate immunity. The actin cytoskeleton is essential for their recruitment and activation in infected tissues. We have previously identified p65/L-plastin with Ca2+-, calmodulin-, and β-actin-binding domains in macrophages. In order to further investigate the p65/L-plastin-involved cellular functions, we cloned the cDNA for murine grancalcin, a possible binding partner of p65/L-plastin. According to the sequence, grancalcin is a member of the penta-EF-hand protein family. We prepared recombinant (r) grancalcin for functional studies and found that it exhibited Ca2+-dependent precipitation. High-titer antibodies against the protein enabled us to detect intracellular grancalcin. A flow cytometric analysis revealed grancalcin to be highly expressed in macrophages and neutrophils. The protein was particularly abundant in those cells recovered from bacteria-infected sites. Immunohistochemical studies clarified that grancalcin was translocated to the actin cytoskeleton in macrophages upon exposure to bacterial lipopolysaccharide. These findings suggest that grancalcin plays a key role in leukocyte-specific functions that are responsible for host defense.

• 社団法人 日本農芸化学会の論文
• 2004-04-23

著者

• KIRIKAE Teruo

  International Medical Center of Japan Research Institute

• Asano Yoshihiro

  Department Of Immunology And Host Defenses Ehime University Graduate School Of Medicine

• Asano Yoshiya

  Department Of Biological And Chemical Engineering Faculty Of Engineering Gunma University

• Kirikae T

  International Medical Center Of Japan Tokyo Jpn

• Kirikae Teruo

  International Medical Center Of Japan

• Kirikae Teruo

  Department Of Ingectious Diseases And Tropical Medicine Research Institute International Center

• Kuratsuji T

  International Medical Center Of Japan

• Asano Yoshihiro

  Department Of Cardiology Osaka University Graduate School Of Medicine

• Teruya Katsuji

  International Medical Center Of Japan

• Kirikae T

  Department Of Infectious Diseases And Tropical Medicine Research Institute International Medical Cen

• Kirikae Teruo

  Department Of Infections Diseases And Tropical Medicine Research Institute International Medical Cen

• Asano Yoshihiro

  Ehime Univ. Graduate School Of Medicine Ehime Jpn

• SHINOMIYA Hiroto

  Department of Immunology and Host Defenses, Ehime University School of Medicine

• LIU Fengzhi

  Department of Immunology and Host Defenses, Ehime University School of Medicine

• HIRATA Hajime

  Department of Life Science, Himeji Institute of Technology

• Shiomiya Hiroto

  Department Of Immunology And Host Defenses Ehime University School Of Medicine

• Shinomiya Hiroto

  Department Of Immunology And Host Defenses Ehime University School Of Medicine

• Liu Fengzhi

  Department Of Immunology And Host Defenses Ehime University School Of Medicine

• Hirata Hajime

  Department Of Life Science Faculty Of Science Hyogo University

• Hasegawa Hiroko

  Department Of Life Science Himeji Institute Of Technology

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