Generation and Characterization of Monoclonal Antibodies That Specifically Recognize p65/L-Plastin Isoform but Not T-Plastin Isoform
スポンサーリンク
概要
- 論文の詳細を見る
The 65-kDa protein (p65) was previously identified as a phosphorylated protein in activated macrophages, and has turned out to be a member of a plastin protein family characterized by a series of Ca2+-, calmodulin-, and β-actin-binding domains. In mice, two isoforms, p65/L-plastin and T-plastin, have so far been identified; p65/L-plastin is expressed in hemopoietic cells and cancer cells, and T-plastin in solid tissue cells. We generated monoclonal antibodies to p65/L-plastin, examined the isoform-specificity by using recombinant (r) T-plastin, and found that the antibodies were specific for rp65/L-plastin, whereas immune sera to rp65/L-plastin showed cross-reactions to rT-plastin. One of the antibodies, p65-7B5, was demonstrated to react to native p65/L-plastin by Western blot, flow cytometric, and immunohistochemical analysis. Furthermore, p65-7B5 has made it possible to detect p65/L-plastin-expressing cells in tissues where T-plastin is abundantly expressed. These reagents and procedures should provide specific tools to investigate the role of p65/L-plastin in leukocytes.
- 社団法人 日本農芸化学会の論文
- 2006-06-23
著者
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KIRIKAE Teruo
International Medical Center of Japan Research Institute
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Asano Yoshihiro
Department Of Immunology And Host Defenses Ehime University Graduate School Of Medicine
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Asano Yoshiya
Department Of Biological And Chemical Engineering Faculty Of Engineering Gunma University
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Kirikae T
International Medical Center Of Japan Tokyo Jpn
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Kirikae Teruo
International Medical Center Of Japan
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Kirikae Teruo
Department Of Ingectious Diseases And Tropical Medicine Research Institute International Center
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Kuratsuji T
International Medical Center Of Japan
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Saito Shouichiro
Department of Anatomy and Embryology, Ehime University School of Medicine
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Asano Yoshihiro
Department Of Cardiology Osaka University Graduate School Of Medicine
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Teruya Katsuji
International Medical Center Of Japan
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Kirikae T
Department Of Infectious Diseases And Tropical Medicine Research Institute International Medical Cen
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Kirikae Teruo
Department Of Infections Diseases And Tropical Medicine Research Institute International Medical Cen
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Asano Yoshihiro
Ehime Univ. Graduate School Of Medicine Ehime Jpn
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TOYOOKA Kiminori
International Medical Center of Japan
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LIU Fenzhi
Department of Immunology and Host Defenses, Ehime University School of Medicine
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ISHII Motoshi
Department of Immunology and Host Defenses, Ehime University School of Medicine
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SHINOMIYA Hiroto
Department of Immunology and Host Defenses, Ehime University School of Medicine
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LIU Fengzhi
Department of Immunology and Host Defenses, Ehime University School of Medicine
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Shiomiya Hiroto
Department Of Immunology And Host Defenses Ehime University School Of Medicine
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Shinomiya Hiroto
Department Of Immunology And Host Defenses Ehime University School Of Medicine
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Liu Fengzhi
Department Of Immunology And Host Defenses Ehime University School Of Medicine
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Ishii Motoshi
Department Of Immunology And Host Defenses Ehime University School Of Medicine
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Saito Shouichiro
Department Of Anatomy And Embryology Ehime University School Of Medicine
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