Generation and Characterization of Monoclonal Antibodies That Specifically Recognize p65/L-Plastin Isoform but Not T-Plastin Isoform
スポンサーリンク
概要
- 論文の詳細を見る
The 65-kDa protein (p65) was previously identified as a phosphorylated protein in activated macrophages, and has turned out to be a member of a plastin protein family characterized by a series of Ca2+-, calmodulin-, and β-actin-binding domains. In mice, two isoforms, p65/L-plastin and T-plastin, have so far been identified; p65/L-plastin is expressed in hemopoietic cells and cancer cells, and T-plastin in solid tissue cells. We generated monoclonal antibodies to p65/L-plastin, examined the isoform-specificity by using recombinant (r) T-plastin, and found that the antibodies were specific for rp65/L-plastin, whereas immune sera to rp65/L-plastin showed cross-reactions to rT-plastin. One of the antibodies, p65-7B5, was demonstrated to react to native p65/L-plastin by Western blot, flow cytometric, and immunohistochemical analysis. Furthermore, p65-7B5 has made it possible to detect p65/L-plastin-expressing cells in tissues where T-plastin is abundantly expressed. These reagents and procedures should provide specific tools to investigate the role of p65/L-plastin in leukocytes.
- 社団法人 日本農芸化学会の論文
- 2006-06-23
著者
-
KIRIKAE Teruo
International Medical Center of Japan Research Institute
-
Asano Yoshihiro
Department Of Immunology And Host Defenses Ehime University Graduate School Of Medicine
-
Asano Yoshiya
Department Of Biological And Chemical Engineering Faculty Of Engineering Gunma University
-
Kirikae T
International Medical Center Of Japan Tokyo Jpn
-
Kirikae Teruo
International Medical Center Of Japan
-
Kirikae Teruo
Department Of Ingectious Diseases And Tropical Medicine Research Institute International Center
-
Kuratsuji T
International Medical Center Of Japan
-
Saito Shouichiro
Department of Anatomy and Embryology, Ehime University School of Medicine
-
Asano Yoshihiro
Department Of Cardiology Osaka University Graduate School Of Medicine
-
Teruya Katsuji
International Medical Center Of Japan
-
Kirikae T
Department Of Infectious Diseases And Tropical Medicine Research Institute International Medical Cen
-
Kirikae Teruo
Department Of Infections Diseases And Tropical Medicine Research Institute International Medical Cen
-
Asano Yoshihiro
Ehime Univ. Graduate School Of Medicine Ehime Jpn
-
TOYOOKA Kiminori
International Medical Center of Japan
-
LIU Fenzhi
Department of Immunology and Host Defenses, Ehime University School of Medicine
-
ISHII Motoshi
Department of Immunology and Host Defenses, Ehime University School of Medicine
-
SHINOMIYA Hiroto
Department of Immunology and Host Defenses, Ehime University School of Medicine
-
LIU Fengzhi
Department of Immunology and Host Defenses, Ehime University School of Medicine
-
Shiomiya Hiroto
Department Of Immunology And Host Defenses Ehime University School Of Medicine
-
Shinomiya Hiroto
Department Of Immunology And Host Defenses Ehime University School Of Medicine
-
Liu Fengzhi
Department Of Immunology And Host Defenses Ehime University School Of Medicine
-
Ishii Motoshi
Department Of Immunology And Host Defenses Ehime University School Of Medicine
-
Saito Shouichiro
Department Of Anatomy And Embryology Ehime University School Of Medicine
関連論文
- Quinolactacins A, B and C : Novel Quinolone Compounds from Penicillium sp. EPF-6 I. Taxonomy, Production, Isolation and Biological Properties
- Nonheme-Iron Deposition in the Hypothalamo-Neurohypophyseal System of the Rat Brain
- PJ-047 The Combined Therapy of Angiotensin Converting Enzyme Inhibitor, Angiotensin II Receptor Blocker and Beta-blockers Submaximally Limits Infarct Size(Acute Myocardial Infarction, Basic 2 (IHD) : PJ8)(Poster Session (Japanese))
- PJ-046 Celiprolol, a Selective β_1-Adrenoceptor Antagonist, Limits Infarct Size via NO-dependent Mechanisms(Acute Myocardial Infarction, Basic 2 (IHD) : PJ8)(Poster Session (Japanese))
- The ATP-sensitive Potassium Channel Opener, Nicorandil, Reduces No-reflow Phenomenon and Infarct Size in the Canine Heart
- OJ-442 Gq-stimulation along with low activity of proteasome induces cardiac apoptosis : Potential mechanism of transition from hypertrophy to failure(Heart Failure, Basic 3 (M) : OJ54)(Oral Presentation (Japanese))
- OJ-324 Antagonism of Aldosterone Receptor Attenuates Severity of Cardiac Dysfunction and Inflammation Induced by Chronic Blockade of NO Synthesis in Rats(Cardiovascular Pharmacology, Basic/Clinical 2 (H) : OJ39)(Oral Presentation (Japanese))
- Canine DNA Array as a Potential Tool for Combining Physiology and Molecular Biology : An Application for the Gene Expression Profile of Regional Ischemic Myocardium
- An Adenosine Analogue, Chloroadenosine, Attenautes Pressure-Overload Myocardial Hypertrophy in Mice
- The New Metalloproteinase Inhibitor Blocks Cardiac Hypertrophy Induced by both Phenylephrine and Angiotensin II
- Decreased Proteasome Activity by Oxidative Stress Induces Cardiac Myocytes Apoptosis via p53-mediated Pathway(Heart Failure, Basic 2 (M), The 69th Annual Scientific Meeting of the Japanese Circulation Society)
- MECHANISMS OF ARCHENTERON FORMATION IN STARFISH EMBRYOGENESIS : DETERMINATION OF VEGETAL POLE CELLS BY WNT/BETA-CATENIN PATHWAY(Developmental Biology,Abstracts of papers presented at the 74^ Annual Meeting of the Zoological Society of Japan)
- Nuclear localization of β-catenin in vegetal pole cells during early embryogenesis of the starfish Asterina pectinifera
- Evaluation of Pharmacogenetic Algorithm for Warfarin Dose Requirements in Japanese Patients
- 3 Altered Epigenetic Memory with Chromatin Disruption in the End-Stage Failing Heart(Young Investigator's Award Finalists Lectures (YIA),Special Program,The 72nd Annual Scientific Meeting of the Japanese Circulation Society)
- Hemostatic effect of tranexamic acid (transamin) during coronary artery bypass grafting
- Protective Effect of OK-432 on Mice against Endotoxemia and Infection with Pseudomonas aeruginosa and Salmonella enteritidis
- HB-EGF Is an Essential Growth Factor for Cardiomyocyte Metabolism : The Possibility for a Novel Therapeutic Target for Heart Failure
- Rho-kinase Inhibitiors Administered After the Onset of Reperfusion Limit Infarct Size : A Novel Strategy for Adjunctive Therapies Against Myocardial Infarction
- A Role of Transient Preischemic Activation of Protein Kinase-A in Ischemic Preconditioning in Vivo : Involvement of p38 Mitogen-activated Protein Kinase
- Molecular Heterogeneity of Heat-Stable Toxin-Producing and Non-Producing Escherichia coli O25 Strains Isolated in Japan
- Amlodipine Increases Coronary Blood Flow and Reduces the Severity of Myocardial Ischemia in Canine Hearts : The Role of NO and Adenosine
- Inhibitory Effects of the CP Compound on Coronary Thromboemboli Caused by an Inhibition of Adenosine Receptors during Coronary Hypoperfusion
- Synthesis of[^3H]-Labeled Bioactive Lipid A Analogs and Their Use or Detection of Liped A-Binding Proteins on Murine Macrophages
- Medical Health Care Checkup Reveals a Novel Risk Factor of Diastolic Heart Dysfunction : Impact of Impaired Glucose Tolerance(Cardiovascular Disease Risk in Diabetes and Glucose Intolerance, The 69th Annual Scientific Meeting of the Japanese Circulation S
- The Long-acting Calcium Channel Blockers Attenuate Cardiac Hypertrophy Induced by Chronic NO Inhibition : The Investigation of the Potential Subcellular Mechanisms
- Gene Expression Profile of Failing Human Hearts In Patients with Dilated Cardiomyopathy Using "Gene Chip" Analysis
- Attenuated Listeria infection activates natural killer cell cytotoxicity to regress melanoma growth in vivo
- Differential Effect of Listeria monocytogenes Infection on Cytokine Production and Cytotoxicity of CD8 T Cells
- Generation and Characterization of Monoclonal Antibodies That Specifically Recognize p65/L-Plastin Isoform but Not T-Plastin Isoform
- Characterization of Murine Grancalcin Specifically Expressed in Leukocytes and Its Possible Role in Host Defense against Bacterial Infection
- Preparation and Characterization of Recombinant Murine p65/L-Plastin Expressed in Escherichia coli and High-titer Antibodies against the Protein(Biochemistry & Molecular Biology)
- A two-step model of T cell subset commitment : antigen-independent commitment of T cells before encountering nominal antigen during pathogenic infections
- Use of Immunoglobulin Enriched Bovine Colostrum against Oral Challenge with Enterohaemorrhagic Escherichia coli O157 : H7 in Mice
- Lipopolysaccharide (LPS)-Induced Intra-Uterine Fetal Death (IUFD) in Mice Is Principally Due to Maternal Cause but Not Fetal Sensitivity to LPS
- Participation of CD14 in the Phagocytosis of Smooth-Type Salmonella typhimurium by the Macrophage-Like Cell Line, J774.1
- Molecular epidemiology of outbreaks and containment of drug-resistant Pseudomonas aeruginosa in a Tokyo hospital
- Emergence of rifampicin resistance in methicillin-resistant Staphylococcus aureus in tuberculosis wards
- "Syndromic Surveillance within a Hospital" for the Early Detection of a Nosocomial Outbreak of Acute Respiratory Infection
- In Situ Characterization of Dendritic Cells Occurring in the Islets of Nonobese Diabetic Mice During the Development of Insulitis
- Evidence-Based Infection Control in Thoracic Surgery
- Modulation of the Immune System by Listeria monocytogenes-Mediated Gene Transfer into Mammalian Cells
- Performance of Pelton-type Turbine Driven by Gas-liquid Two-phase Flow : 1st Report, Experimental Study of Fundamental Characteristics
- Optical Absorption Spectra of Nickel Ion in Alkali Halide Crystals in the Ultraviolet Region
- An alternate pathway for type 1 T cell differentiation
- Electron Spin Resonance Spectra of Nickel Ion in Alkali Halide Crystals
- Detection of the Activities of Multiple Protein Kinases in Lipopolysaccharide-Stimulated Macrophages by Renaturation
- Analysis of a Bacterial Lipopolysaccharide-Activated Serine Kinase That Phosphorylates p65/L-Plastin in Macrophages
- New Sulfoalkylresorcinol from Marine-derived Fungus, Zygosporium sp. KNC52
- Nonocclusive mesenteric ischemia after cardiopulmonary bypass
- Flagellin as a Biomarker for Bacillus subtilis Strains; Application to the DB9011 Strain and the Study of Interspecific Diversity in Amino-acid Sequences
- Establishment of Monoclonal Antibodies Specific for Bacillus subtilis DB9011
- <Poster>Cellular and subcellular localizations of nonheme ferric and ferrous iron in the rat brain : a light and electron microscopic study by the perfusion-Perls and -Turnbull methods
- Cellular and subcellular localizations of nonheme ferric and ferrous iron in the rat brain : a light and electron microscopic study by the perfusion-Perls and -Turnbull methods
- Chelatable Fe (II) is generated in the rat kidneys exposed to ischemia and reperfusion, and a divalent metal chelator, 2, 2'-dipyridyl, attenuates the acute ischemia/reperfusion-injury of the kidneys : a histochemical study by the perfusion-Perls and -Tur
- The Impact of Cardiac Resynchronization Therapy in an End-Stage Heart Failure Patient With a Left Ventricular Assist Device as a Bridge to Recovery : A Case Report
- Antibiotic-Induced Endotoxin Release from Bacteria and Its Clinical Significance
- Sildenafil and steroid therapy effectively improved POEMS syndrome-associated pulmonary arterial hypertension
- Differential Expression of Histochemical Characteristics in the Developing Olfactory Receptor Cells in a Flatfish, Barfin Flounder (Verasper moseri)
- Expression Patterns of Glycoconjugates in the Three Distinctive Olfactory Pathways of the Clawed Frog, Xenopus laevis.
- Morphogenesis of the Olfactory Pit in a Flatfish, Barfin Flounder (Verasper moseri)
- Development of Duplex PCR Assay for Detection and Differentiation of Typical and Atypical Melissococcus plutonius Strains