Simplified Transposon Display(STD) : a New Procedure for Isolation of a Gene Tagged by a Transposable Element Belonging to the Tpn1 Family in the Japanese Morning Glory

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概要

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• Transposable elements are regarded as a powerful mutagen and as an effective tool to isolate genes tagged by transposon insertions. In the Japanese morning glory, a number of spontaneous mutants related to the colors and shapes of the flowers have been isolated. The plant contains around 500-1000 copies of an En/Spm-related element Tpn1 and its relatives, which act as major spontaneous mutagens. We have developed a new protocol for identifying genes tagged by insertion of Tpn1-related elements. The procedure, named simplified transposon display (STD), is simple and requires neither biotinylated oligonucleotides nor streptavidin-capturing which are essential in other transposon display methods published recently. Here we describe the details of STD used for identification of the Purple(Pr) gene that encodes a vacuolar Na+/H+ exchanger for increasing vacuolar pH responsible for blue flower coloration.

• 日本植物細胞分子生物学会の論文
• 2001-06-01

著者

• IIDA Shigeru

  National Institute for Basic Biology

• Iida S

  National Inst. For Basic Biology

• Iida Shigeru

  National Institute For Basic Biology Okazaki

• Fukuda Tanaka

  National Institute For Basic Biology

• INAGAKI Yoshishige

  National Institute for Basic Biology

• Fukada-Tanaka Sachiko

  National Institute for Basic Biology

• Iida S

  National Institute For Basic Biology

• Iida Shigeru

  National Inst. For Basic Biology

• YAMAGUCHI Toshio

  Department of Physics, Tokyo Women's Medical University

• YAMAGUCHI Toshio

  National Institute for Basic Biology

• Inagaki Y

  National Institute For Basic Biology

• Yamaguchi Toshio

  Department Of Chemistry Faculty Of Science Fukuoka University

• Yamaguchi Toshio

  Department of Chemical Engineering, Tokyo University of Agriculture and Technology

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