Examination of transpositional activity of nDart1 at different stages of rice development

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概要

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• As a useful tool to elucidate gene functions, a rice transposon tagging line has been developed using an active endogenous DNA transposon, nDart1. It was highly desirable to evaluate the transposition timing and frequency of the nDart1 elements during rice development to facilitate the generation of an efficient mutant isolation system. Comparison of the detected new insertions at different stages of rice development by transposon display analysis demonstrated that the last heading tiller carry a higher number of nDart1 elements than the main culm. Moreover, it was revealed that the last heading tiller could produce progeny that carried more new insertions of nDart1 elements, mainly as a result of the accumulation of somatic insertions in the parental plant. This report demonstrates that late tillers increase the probability of producing independent mutant lines.

• 2011-06-25

著者

• Tsugane Kazuo

  National Inst. For Basic Biology

• Tsugane Kazuo

  National Institute For Basic Biology

• IIDA Shigeru

  National Institute for Basic Biology

• Maekawa Masahiko

  Institute of Plant Science and Resources, Okayama University

• Iida Shigeru

  National Inst. For Basic Biology

• Maekawa Masahiko

  Institute Of Plant Science And Resources Okayama University

• Hayashi-Tsugane Mika

  National Institute for Basic Biology

• Kobayashi Hirokazu

  Graduate School Of Frontier Sciences The University Of Tokyo

• Iida Shigeru

  Graduate School Of Pharmaceutical Sciences Univ. Of Shizuoka

• Kobayashi Hirokazu

  Graduate School Of Nutritional And Environmental Sciences University Of Shizuoka

• HAYASHI TSUGANE

  National Institute for Basic Biology

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