Natriuretic Peptide Receptors, NPR-A and NPR-B, in CulturedRabbit Retinal Pigment Epithelium Cells
スポンサーリンク
概要
- 論文の詳細を見る
We tried to detect natriuretic peptide (NP) receptor (NPR-A and NPR-B) mRNAs in cultured rabbit retinal pigment epithelium (RPE) cells and examined the regulation of their expression in relation to subretinal fluid absorption or RPE cell proliferation. RPE cells from 2 - 4 passages were grown to confluence on microporous membranes and analyzed for levels of expression of receptor mRNAs by quantitative RT-PCR and Northern blotting. The expression of NPR-B mRNA was approximately tenfold higher than that of NPR-A mRNA. The expression of NPR-A mRNA was not affected by treatments that may change subretinal fluid transport, while that of NPR-B mRNA was inhibited by transmitters involved in light- and dark-adaptation such as dopamine and melatonin. Expression of NPR-B mRNA was also suppressed by platelet-derived growth factor and transforming growth factor-β. Furthermore, atrial natriuretic peptide (ANP) and C-type natriuretic peptide (CNP), ligands for NPR-A and B, respectively, inhibited the proliferation of RPE cells, as analyzed by incorporation of [3H]thymidine. These findings suggest that ANP may be involved in constitutive absorption of subretinal fluid and that NPs form an important regulatory system of proliferation in RPE cells.
- 社団法人 日本薬理学会の論文
- 1999-03-01
著者
-
UYAMA Masanobu
Department of Ophthalmology, Kansai Medical University
-
Inagaki Chiyoko
Department of Pharmacology, Kansai Medical University, Japan
-
Uyama M
Departments Ophthatmology Kansai Medical University
-
Uyama Masanobu
Department Of Ophthalmology Kansai Medical University
-
Inagaki C
Kansai Medical Univ. Osaka Jpn
-
Inagaki Chiyoko
Department Of Biology Kyoto College Of Pharmacy
-
MIKAMI Yoshito
Department of Pharmacology
-
OMORI Kyoko
Department of Pharmacology
-
Omori Koichiro
Department Of Physiology Kansai Medical University
-
Okugawa Gaku
Departments Of Pharmacology Physiology And Ophthalmology Kansai Medical University
-
Mikami Yoshito
Departments Ophthatmology Kansai Medical University
-
FUJISEKI Yoshito
Departments of Pharmacology Physiology and Ophthalmology, Kansai Medical University
-
SUZUKAWA Junko
Departments of Pharmacology Physiology and Ophthalmology, Kansai Medical University
-
Omori Kyoko
Department Of Physiology Kansai Medical University
-
Suzukawa Junko
Departments Of Pharmacology Physiology And Ophthalmology Kansai Medical University
-
Fujiseki Yoshito
Departments Of Pharmacology Physiology And Ophthalmology Kansai Medical University
-
INAGAKI Chiyoko
Departmctit of Pharmacology, Faculty of Medicine, Kyoto University
関連論文
- Morphologic characteristics of N-methyl-N-nitrosourea-induced retinal degeneration in C_ BL mice
- Involvement of Protein Kinase C and RhoA in Protease-Activated Receptor 1–Mediated F-Actin Reorganization and Cell Growth in Rat Cardiomyocytes
- Multicenter Genetic Study of Retinitis Pigmentosa in Japan : I. Genetic Heterogeneity in Typical Retinitis Pigmentosa
- Multicenter Genetic Study of Retinitis Pigmentosa in Japan : II. Prevalence of Autosomal Recessive Retinitis Pigmentosa
- Perospirone for treatment of dissociative identity disorder
- Expression of Transforming Growth Factor-β Receptors in Normal Rat Retina and Experimental Choroidal Neovascularization
- Adrenergic Receptor-Mediated Cl^- Transport in Rabbit Corneal Endothelial Cells
- GENE EXPRESSIONS OF BASIC FIBROBLAST GROWTH FACTOR AND ITS RECEPTOR IN HEALING OF RAT RETINA AFTER LASER PHOTOCOAGULATION
- IMMUNOHISTOCHEMICAL PROOF OF ORIGIN OF MACROPHAGES IN LASER PHOTOCOAGULATION LESION IN THE RETINA
- Effects of Local Administration of Interferon-β on Proliferation of Retinal Pigment Epithelium in Rabbit After Laser Photocoagulation
- FGF RECEPTOR 1 EXPRESSION IN EXPERIMENTAL CHOROIDAL NEOVASCULARIZATION
- Immunocytochemical Analyses of Distributions of Na, K-ATPase and GLUT1 Insulin and Transferrin Receptors in the Developing Retinal Pigment Epithelial Cells
- Comparative Study of Experimental Choroidal Neovascularization by Optical Coherence Tomography and Histopathology
- GABA_C-Receptor Stimulation Activates cAMP-Dependent Protein Kinase via A-Kinase Anchoring Protein 220
- Molecular Mechanisms for α_2-Adrenoceptor-Mediated Regulation of Synoviocyte Populations
- Phorbol 12-Myristate 13-Acetate(PMA)-Induced Oxyradical Production in Rheumatoid Cells
- A 60 kDa Plasma Membrane Protein Changes its Localization to Autophagosome and Autolysosome Membranes during Induction of Autophagy in Rat Hepatoma Cell Line, H-4-II-E Cells
- Natriuretic Peptide Receptors, NPR-A and NPR-B, in CulturedRabbit Retinal Pigment Epithelium Cells
- Effects of Growth Hormone on the Differentiation of Mouse B-Lymphoid Precursors
- Immunocytochemical Localization of Na, K-ATPase in Rat Muscle Spindles
- IMMUNOHISTOCHEMICAL PROOF OF INTRANEURAL LOCALIZATION OF HERPES SIMPLEX VIRUS IN EXPERIMENTAL RETINITIS
- Immunohistochemical Demonstration of CI^- Pump in Type A Intercalated Cells of Rat Kidney
- POSSIBLE MECHANISMS OF THE TRIPHASIC EFFECTS OF NEUROTENSIN ON THE RAT BLOOD PRESSURE
- UPTAKE OF 14C-LABELLED 5-HYDROXYTRYPTOPHAN AND 5-HYDROXYTRYPTAMINE BY SEPARATED RAT RENAL TUBULES
- INHIBITORY EFFECT OF (+)THREO-3, 4-DIHYDROXY-PHENYLSERINE (DOPS) ON DECARBOXYLATION OF (-)THREO-DOPS
- INHIBITORY EFFECTS OF D-ISOMERS OF AROMATIC AMINO ACIDS ON THE DECARBOXYLATION OF L-5-HYDROXYTRYPTOPHAN
- NEONATAL AND SENESCENT CHANGES IN L-AROMATIC AMINO ACID DECARBOXYLASE AND MONOAMINE OXIDASE ACTIVITIES IN KIDNEY, LIVER, BRAIN AND HEART OF THE RAT
- EFFECTS OF DIAZEPAM, NITRAZEPAM AND BROTIZOLAM ON DOPAMINE TURNOVER IN THE OLFACTORY TUBERCLE, NUCLEUS ACCUMBENS AND CAUDATE NUCLEUS OF RATS
- Comparison of Indocyanine Green and Fluorescein Angiography of Choroidal Neovascularization
- RAPID PURIFICATION OF MONOCLONAL ANTIBODY IN ASCITES BY HIGH PERFORMANCE ION EXCHANGE COLUMN CHROMATOGRAPHY FOR DIMINISHING NON-SPECIFIC STAINING
- Na, K-, Mg- AND HCO3-ADENOSINE TRIPHOSPHATASES IN THE RABBIT BRAIN CHOROID PLEXUS
- ENZYMATIC DECARBOXYLATION OF L-THREO-3, 4-DIHYDROXYPHENYLSERINE IN RAT HEART
- Involvement of Protein Kinase C and RhoA in Protease-Activated Receptor 1-Mediated F-Actin Reorganization and Cell Growth in Rat Cardiomyocytes
- COMPARISON BETWEEN FLUORIMETRIC AND RADIOCHEMICAL ASSAYS OF IONTOPHORETICALLY RELEASED NORADRENALINE FROM A SEVEN-BARRELED MICROPIPETTE
- EFFECT OF ALDOSTERONE ON RENAL Na, K-ACTIVATED ADENOSINE-TRIPHOSPHATASE ACTIVITY IN NON-ADRENALECTOMIZED RATS
- Sensitization to heat by amiloride analogues in Chinese hamster cells.
- GABAC-Receptor Stimulation Activates cAMP-Dependent Protein Kinase via A-Kinase Anchoring Protein 220
- Electronmicroscopical Studies on Retina in Stroke-Prone SHR
- Purification and Characterization of Neurotrophic Factor for Retinal Cholinergic Neurons Derived from Cultured Hippocampal Neurons.
- Characteristics of ethacrynic acid highly sensitive Mg2+-ATPase in microsomal fractions of the rat brain : Functional molecular size, inhibition by SITS and stimulation by Cl-.
- Solubilization and separation of ethacrynic acid(EA) highly sensitive and EA less sensitive Mg2+-ATPases in the rat brain.
- Ethacrynic Acid-Sensitive and ATP-Dependent Cl- Transport in the Rat Kidney.