トリメチルアミンN-オキサイドで誘導した大腸菌の膜におけるb型およびc型チトクロムの存在

概要

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The presence of b- and c-type cytochromes has been examined in membrane fragments from Escherichia coli grown anaerobically in the presence of trimethylamine N-oxide (TMO) (TMO-induced cells). The amounts of both the cytochromes in the membrane were compared with those of cells grown in the presence of nitrate (nitrate-induced cells). After the membrane fragments were treated with acid-acetone, pyridine hemochrome spectra were taken of the fractions soluble and insoluble in acid-acetone. The spectra of the soluble fractions from both TMO- and nitrateinduced cells had typical protoheme peaks with the maxima at 556nm, 525nm, and 420nm, suggesting the presence of b-type cytochromes. The amount of protoheme in TMO-induced cells was much smaller than that in nitrate-induced cells. The spectra of the insoluble fraction from TMO-induced cells only, contained the peaks of heme c with the maxima at 550.5nm, 521nm, and 415nm, indicating the presence of c-type cytochromes in the membrane. The estimated heme c concentration was about 0.4nmol/mg protein. The roles of the b- and c-type cytochromes are discussed in relation to the electron transport system of TMO reduction in E. coli.
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