Sub-micro-liter Electrochemical Single-Nucleotide-Polymorphism Detector for Lab-on-a-Chip System
スポンサーリンク
概要
- 論文の詳細を見る
A sub-micro-liter single-nucleotide-polymorphism (SNP) detector for lab-on-a-chip applications is developed. This detector enables a fast, sensitive, and selective SNP detection directly from human blood. The detector is fabricated on a Si substrate by a standard complementary metal oxide semiconductor/micro electro mechanical systems (CMOS/MEMS) process and Polydimethylsiloxane (PDMS) molding. Stable and reproducible measurements are obtained by implementing an on-chip Ag/AgCl electrode and encapsulating the detector. The detector senses the presence of SNPs by measuring the concentration of pyrophosphoric acid generated during selective DNA amplification. A 0.5-μL-volume detector enabled the successful performance of the typing of a SNP within the ABO gene using human blood. The measured sensitivity is 566 pA/μM.
- 2012-04-25
著者
-
Yamashita Ichiro
Advanced Technology Research Laboratories Matsushita Electric Industrial Co. Ltd.
-
Hayashi Miho
R&D Center, Panasonic Healthcare Corporation, Toon, Ehime 791-0395, Japan
-
Tanaka Hiroyuki
Advanced Technology Research Laboratories, Panasonic Corporation, Seika, Kyoto 619-0237, Japan
-
Sterken Tom
IMEC vwz., Kapeldreef 75, B-3001 Leuven, Belgium
-
Fiorini Paolo
IMEC vwz., Kapeldreef 75, B-3001 Leuven, Belgium
-
Yamashita Ichiro
Advanced Technology Research Laboratory, Panasonic Corporation, Seika, Kyoto 619-0237, Japan
-
Peeters Sara
IMEC vwz., Kapeldreef 75, B-3001 Leuven, Belgium
-
Majeed Bivragh
IMEC vwz., Kapeldreef 75, B-3001 Leuven, Belgium
-
Beeck Maaike
IMEC vwz., Kapeldreef 75, B-3001 Leuven, Belgium
-
Hayashi Miho
R&D Center, Panasonic Healthcare Corporation, Toon, Ehime 791-0395, Japan
-
Yaku Hidenobu
Advanced Technology Research Laboratory, Panasonic Corporation, Seika, Kyoto 619-0237, Japan
関連論文
- Floating Gate MOS Capacitor with High-Density Nanodots Array Produced by Protein Supramolecule
- High-Density Floating Nanodots Memory Produced by Cage-Shaped Protein
- Making Monolayer of Inorganic Nanoparticles on Silicon Substrate
- Encapsulation of Ferritin within a Hollow Cylinder of Glycolipid Nanotubes
- Stochastic Associative Processor Operated by Random Voltages(LSI Applications,Fundamentals and Applications of Advanced Semiconductor Devices)
- Bio-template Synthesis of Uniform CdSe Nanoparticles Using Cage-shaped Protein, Apoferritin
- Direct Production of a Two-Dimensional Ordered Array of Ferritin-Nanoparticles on a Silicon Substrate
- Ordered Array of Gold Nanoparticles Promoted by Functional Peptides
- Position-Controlled Vertical Growths of Individual Carbon Nanotubes Using a Cage-Shaped Protein
- NOR-Type Nonvolatile Ferroelectric-Gate Memory Cell Using Composite Oxide Technology
- Silicon Based System for Single-Nucleotide-Polymorphism Detection : Chip Fabrication and Thermal Characterization of Polymerase Chain Reaction Microchamber (Special Issue : Solid State Devices and Materials (2))
- Sub-micro-liter Electrochemical Single-Nucleotide-Polymorphism Detector for Lab-on-a-Chip System
- Floating Gate Metal–Oxide–Semiconductor Capacitor Employing Array of High-Density Nanodots Produced by Protein Supramolecule
- High-Density and Highly Surface Selective Adsorption of Protein–Nanoparticle Complexes by Controlling Electrostatic Interaction
- Low-temperature Polycrystalline Silicon Thin Film Transistor Flash Memory with Ferritin
- Effects of Dot Density and Dot Size on Charge Injection Characteristics in Nanodot Array Produced by Protein Supramolecules
- Novel Method for Making Nanodot Arrays Using a Cage-like Protein
- Electrostatic Placement of Nanodots onto Silicon Substrate Using Ferritin Protein Supramolecules with Control of Electrostatic Interaction in Solution