Image-Based Identification of Single Neurons for Noninvasive Imaging Purification
スポンサーリンク
概要
- 論文の詳細を見る
A single-cell-based screening assay requires strict identification and isolation of particular target cells from a mixture of various kinds of cells. We have developed a visual-image-based on-chip microfluidic cell sorting method for the collection of neurons. One of the advantages of our method of purifying neurons is the direct monitoring and reorganization of neurons with specific image indexes, such as the cell size, shape, internal complexity, and spatial distribution of a fluorescent dye of a specific antibody marker by phase-contrast/fluorescence microscopy and image processing, which has not been realized using conventional diffraction-based cell sorting systems. First, we compared the differences of microscopic images (shapes) of neurons and glia cells, and found that only neurons have neurites extending from the cell body. We also found that the smooth surface shape indicates neurons, and the rough surface shape indicates glia cells. After picking the neuron cells manually chosen by observing their shapes as described above, we confirmed that the purified neurons can be cultivated and can keep their electrophysiological functions on the chip even after the purification procedure. The results indicate the potential of a nonlabel, noninvasive on-chip cell sorting procedure for neurons using micrograph images for an on-chip ultrahigh-speed camera-based imaging cell sorter.
- Published by the Japan Society of Applied Physics through the Institute of Pure and Applied Physicsの論文
- 2011-06-25
著者
-
Hattori Akihiro
Kanagawa Academy of Science and Technology
-
Hayashi Masahito
Kanagawa Academy Of Science And Technolgoy
-
Yasuda Kenji
Kanagawa Academy Of Science And Technology
-
Kim Hyonchol
Kanagawa Academy of Science and Technology
-
Terazono Hideyuki
Kanagawa Academy of Science and Technology
-
Kaneko Tomoyuki
Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University
-
Kim Hyonchol
Kanagawa Academy of Science and Technology, Kawasaki 213-0012, Japan
-
Hattori Akihiro
Kanagawa Academy of Science and Technology, Kawasaki 213-0012, Japan
-
Terazono Hideyuki
Kanagawa Academy of Science and Technology, Kawasaki 213-0012, Japan
関連論文
- 1P-315 画像認識型セルソーティングシステムの開発(2) : 細胞イメージのための被写界深度と分解能の改良(バイオエンジニアリング,第46回日本生物物理学会年会)
- 2P318 1細胞レベル分子発現解析を目指した「多色」ナノ粒子標識法の開発(計測,第48回日本生物物理学会年会)
- 3P-337 高輝度LEDを用いた超高速顕微観察用パルス光源の開発(バイオイメージング(2),第46回日本生物物理学会年会)
- 3P339 超高速画像解析による細胞分析・分離装置の開発(バイオエンジニアリング,第48回日本生物物理学会年会)
- 3P-241 2神経細胞の自発活動ダイナミクス(神経回路・脳の情報処理,第46回日本生物物理学会年会)
- 3P-192 オンチップ心筋ネットワークにおける細胞外電位の異方性伝導(細胞生物学的課題(3),第46回日本生物物理学会年会)
- 2P493 Development of a novel cell sorting system based on image recognition and microfabrication technology (III) On-chip cell recultivation(51. New methods and tools (II),Poster Session,Abstract,Meeting Program of EABS & BSJ 2006)
- 2P492 Development of a novel cell sorting system based on image recognition and microfabrication technology (II) : analysis based cell separation(51. New methods and tools (II),Poster Session,Abstract,Meeting Program of EABS & BSJ 2006)
- 2P491 Development of a novel cell sorting system based on image recognition and microfabrication technology (I) : System development(51. New methods and tools (II),Poster Session,Abstract,Meeting Program of EABS & BSJ 2006)
- 2P380 On-chip regulation of neural stem cell differentiation(44. Neuro-biophysics,Poster Session,Abstract,Meeting Program of EABS & BSJ 2006)
- 2P379 Plasticity in single-cell-based reconstructed neuronal network pattern(44. Neuro-biophysics,Poster Session,Abstract,Meeting Program of EABS & BSJ 2006)
- 1P405 Study of Cellular Communication of Suprachiasmatic Nucleus through Synaptic Connections(16. Cell-cell communication,Poster Session,Abstract,Meeting Program of EABS & BSJ 2006)
- 2P211 Community effect to the drug sensitivity on geometrical patterned cardiomyocytes network by using On-Chip MEA system(The 48th Annual Meeting of the Biophysical Society of Japan)
- 2P-154 多電極チップを用いた心筋細胞小集団による長期的心毒性評価法の開発(細胞生物的課題(接着,運動,骨格,伝達,膜),第47回日本生物物理学会年会)
- 2P-153 オンチップ多電極電位計測システムを用いたマウス胎仔心筋細胞における細胞外電位の波形解析(細胞生物的課題(接着,運動,骨格,伝達,膜),第47回日本生物物理学会年会)
- 1P125 Fixed interaction between the Escherichia coli RuvA and RuvB proteins during branch migration of Holliday junction(Nucleic acid binding proteins,The 48th Annual Meeting of the Biophysical Society of Japan)
- 1P297 Direct observation of T7 RNA polymerase rotation during transcription.(9. Molecular motor (I),Poster Session,Abstract,Meeting Program of EABS & BSJ 2006)
- 2P210 電気刺激による心筋細胞ネットワークのコミュニティ・エフェクトの解析(細胞生物的課題(接着,運動,骨格,伝達,膜),第48回日本生物物理学会年会)
- Production of nanoparticles using several materials for labeling of biological molecules
- 2P-257 超高速リアルタイムPCR装置を用いた非特異的DNA断片増幅の抑制(計測,第47回日本生物物理学会年会)
- 2TA3-08 走査型電子顕微鏡における「多色」標識法(計測,第47回日本生物物理学会年会)
- 3P-317 走査型電子顕微鏡観察用金属ラベル : 元素別識別方法(計測,第46回日本生物物理学会年会)
- 3P-304 様々な粒径・元素のナノ粒子プローブセットで標識された標的DNAの無増幅定量検出法の開発(計測,第46回日本生物物理学会年会)
- 2P-138 マクロファージによる逐次貪食過程の観察(細胞生物的課題(接着,運動,骨格,伝達,膜),第47回日本生物物理学会年会)
- Improvement of particle alignment control and precise image acquisition for on-chip high-speed imaging cell sorter (Special issue: Microprocesses and nanotechnology)
- Development of a High-Speed Real-Time Polymerase Chain Reaction System Using a Circulating Water-Based Rapid Heat-Exchange
- 2P-155 多電極計測システムを用いた心筋細胞ネットワークの集団化効果の理解(細胞生物的課題(接着,運動,骨格,伝達,膜),第47回日本生物物理学会年会)
- Comprehensive Study of Microgel Electrode for On-Chip Electrophoretic Cell Sorting
- Simple Microfluidic Continuous Concentration of Microparticles with Different Dielectric Constants Using Dielectrophoretic Force in a V-Shaped Electrode Array
- Image-based identification of single neurons for noninvasive imaging purification (Special issue: Microprocesses and nanotechnology)
- Production of double-layered metal nanocups for artificial nanospace of biomolecular reaction (Special issue: Microprocesses and nanotechnology)
- Continuous concentration and separation of microparticles using dielectrophoretic force in a V-shaped electrode array (Special issue: Microprocesses and nanotechnology)
- 2J1612 V字型電極アレイを用いた誘電泳動力による微粒子の連続的濃縮分離(計測、その他,第49回日本生物物理学会年会)
- 2N1612 画像認識によるフローサイトメトリーのための被写界深度拡大光学系の開発(バイオイメージング2,第49回日本生物物理学会年会)
- 3J0948 生体反応過程を再構成する人工ナノ空間を模した様々なサイズ・元素のヤヌス粒子とカップの作製(3J バイオエンジニアリング1,日本生物物理学会第49回年会)
- 3F1346 非侵襲的な細胞精製に向けた画像認識による神経細胞の同定と測定技術の開発(3F 神経・感覚、神経回路・脳の情報処理,日本生物物理学会第49回年会)
- 2J1424 オンチップ細胞計測技術を用いた薬剤の心毒性評価システムの開発(計測、その他,第49回日本生物物理学会年会)
- 2J1436 オンチップ多電極計測システムを用いたヒトES細胞由来心筋細胞塊の細胞外電位計測(計測、その他,第49回日本生物物理学会年会)
- 2J1448 Quasi-in vivo heart electrocardiogram measurement using convolution of field potential propagation in cardiomyocyte network circuit(Measurements, Miscellaneous topics,The 48th Annual Meeting of the Biophysical Society of Japan)
- Highly Sensitive Detection of Target Biomolecules on Cell Surface Using Gold Nanoparticle Conjugated with Aptamer Probe (Special Issue : Microprocesses and Nanotechnology)
- Importance of Thickness in Human Cardiomyocyte Network for Effective Electrophysiological Stimulation Using On-Chip Extracellular Microelectrodes (Special Issue : Microprocesses and Nanotechnology)
- Production of Nanoparticles Using Several Materials for Labeling of Biological Molecules
- Cell-Sorting System with On-Chip Imaging for Label-Free Shape-Based Selection of Cells
- Continuous Concentration and Separation of Microparticles Using Dielectrophoretic Force in a V-Shaped Electrode Array
- Production of Double-Layered Metal Nanocups for Artificial Nanospace of Biomolecular Reaction
- 2D1510 インビトロ心毒性予測のための心筋細胞ネットワークの時間的細胞外電位ゆらぎと空間的伝達速度ゆらぎの評価(計測,口頭発表,日本生物物理学会第50回年会(2012年度))
- Non-amplified Quantitative Detection of Nucleic Acid Sequences Using a Gold Nanoparticle Probe Set and Field-Emission Scanning Electron Microscopy
- Image-Based Identification of Single Neurons for Noninvasive Imaging Purification
- Physiological Sample Uniformity and Time--Course Stability in Lined-Up Structure of Human Cardiomyocyte Network for In vitro Predictive Drug-Induced Cardiotoxicity
- Temperature-Shift Speed Dependence of Nonspecific Amplification of Polymerase Chain Reaction Examined by 1480 nm Photothermal Transition Speed Controllable High-Speed Polymerase Chain Reaction System
- Improvement of Particle Alignment Control and Precise Image Acquisition for On-Chip High-Speed Imaging Cell Sorter
- 1P316 明視野/蛍光画像の同時リアルタイム解析技術を用いたオンチップ・マルチイメージング・フローサイトメーターの開発(28. バイオエンジニアリング,ポスター,日本生物物理学会年会第51回(2013年度))