Comprehensive Study of Microgel Electrode for On-Chip Electrophoretic Cell Sorting
スポンサーリンク
概要
- 論文の詳細を見る
We have developed an on-chip cell sorting system and microgel electrode for applying electrostatic force in microfluidic pathways in the chip. The advantages of agarose electrodes are 1) current-driven electrostatic force generation, 2) stability against pH change and chemicals, and 3) no bubble formation caused by electrolysis. We examined the carrier ion type and concentration dependence of microgel electrode impedance, and found that CoCl2 has less than 1/10 of the impedance from NaCl, and the reduction of the impedance of NaCl gel electrode was plateaued at 0.5 M. The structure control of the microgel electrode exploiting the surface tension of sol-state agarose was also introduced. The addition of 1% (w/v) trehalose into the microgel electrode allowed the frozen storage of the microgel electrode chip. The experimental results demonstrate the potential of our system and microgel electrode for practical applications in microfluidic chips.
- 2010-06-25
著者
-
Hattori Akihiro
Kanagawa Academy of Science and Technology
-
Yasuda Kenji
Kanagawa Academy Of Science And Technology
-
Akihiro Hattori
Kanagawa Academy of Science and Technology, KSP East 310, 3-2-1 Sakado, Takatsu, Kawasaki 213-0012, Japan
関連論文
- 1P-315 画像認識型セルソーティングシステムの開発(2) : 細胞イメージのための被写界深度と分解能の改良(バイオエンジニアリング,第46回日本生物物理学会年会)
- 2P318 1細胞レベル分子発現解析を目指した「多色」ナノ粒子標識法の開発(計測,第48回日本生物物理学会年会)
- 3P-337 高輝度LEDを用いた超高速顕微観察用パルス光源の開発(バイオイメージング(2),第46回日本生物物理学会年会)
- 3P339 超高速画像解析による細胞分析・分離装置の開発(バイオエンジニアリング,第48回日本生物物理学会年会)
- 3P-241 2神経細胞の自発活動ダイナミクス(神経回路・脳の情報処理,第46回日本生物物理学会年会)
- 3P-192 オンチップ心筋ネットワークにおける細胞外電位の異方性伝導(細胞生物学的課題(3),第46回日本生物物理学会年会)
- 2P493 Development of a novel cell sorting system based on image recognition and microfabrication technology (III) On-chip cell recultivation(51. New methods and tools (II),Poster Session,Abstract,Meeting Program of EABS & BSJ 2006)
- 2P492 Development of a novel cell sorting system based on image recognition and microfabrication technology (II) : analysis based cell separation(51. New methods and tools (II),Poster Session,Abstract,Meeting Program of EABS & BSJ 2006)
- 2P491 Development of a novel cell sorting system based on image recognition and microfabrication technology (I) : System development(51. New methods and tools (II),Poster Session,Abstract,Meeting Program of EABS & BSJ 2006)
- 2P379 Plasticity in single-cell-based reconstructed neuronal network pattern(44. Neuro-biophysics,Poster Session,Abstract,Meeting Program of EABS & BSJ 2006)