Physiological Sample Uniformity and Time--Course Stability in Lined-Up Structure of Human Cardiomyocyte Network for In vitro Predictive Drug-Induced Cardiotoxicity
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概要
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We have evaluated the electrophysiological characteristics of a line-shaped network of a three-dimensionally controlled in vitro human cardiomyocyte assay (hCM line) against conventional cell clusters as the standard model (hCM cluster) from the viewpoint of quality control of sample variety and time--course stability. The beating intervals of the hCM line demonstrated a more stable uniformity of samples (846 \pm 130 ms, 15.3% fluctuation) and better time--course stability, whereas those of the hCM cluster showed a much larger variety of samples (2001 \pm 1127 ms, 56.3% fluctuation) and weaker time--course stability. The field potential amplitude of the hCM line also showed better uniformity of samples (629 \pm 428 μV, 68.0% fluctuation) against those of the hCM cluster (1984 \pm 2288 μV, 115.3% fluctuation). The results suggested the importance of the cell-network shape control for the uniformity and stability of the beating interval and the field potential amplitude. They also suggest that the hCM line can improve the reproducibility and accuracy of the samples, which is important for a functional human cardiotoxicity model.
- 2013-06-25
著者
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Yasuda Kenji
Institute of Biomaterials and Bioengineering Dept. of Biomedical Information, Tokyo Medical and Dent
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Kaneko Tomoyuki
Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University
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Nomura Fumimasa
Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University
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Kaneko Tomoyuki
Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, Chiyoda, Tokyo 101-0062, Japan
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Hamada Tomoyo
Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University
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Hamada Tomoyo
Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, Chiyoda, Tokyo 101-0062, Japan
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Nomura Fumimasa
Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, Chiyoda, Tokyo 101-0062, Japan
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