Molecular Commonality Sensing of Phosphoric Anhydride Substances Using an Ion-Sensitive Field-Effect Transistor Covered with an Artificial Enzyme Membrane
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概要
- 論文の詳細を見る
A new type of biosensor has been developed using an artificial enzyme membrane that recognizes and hydrolyzes phosphoric anhydride bonds selectively. The artificial enzyme (artificial P–P hydrolase) membrane was designed and synthesized by means of our original procedure, and mounted on a T2O5-gate ion-sensitive field-effect transistor (ISFET). H2PO4- ions are produced through the catalytic reaction in the artificial P–P hydrolase membrane, and accumulate in the membrane on the ISFET. The accumulated H2PO4- ions cause a shift in the static ISFET output as the sensor response. A key advantage of the artificial P–P hydrolase is its specific structure selectivity for the phosphoric anhydride bonds, which is one of the dominant and characteristic structures in biological energy substances. The molecular commonality detection of the phosphoric anhydride bonds is a novel tactic for biosurveillance and cellular viability assays.
- Published by the Japan Society of Applied Physics through the Institute of Pure and Applied Physicsの論文
- 2007-11-15
著者
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OZASA Kazunari
RIKEN (The Institute of Physical and Chemical Research)
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Haruyama Tetsuya
Department Of Applied Biological Science Faculty Of Science And Technology Tokyo University Of Scien
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Migita Satoshi
Department Of Biological Functions And Engineering Kyushu Institute Of Technology
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Ikeno Shinya
Department Of Biological Functions And Engineering Kyushu Instiute Of Technology
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Haruyama Tetsuya
Department of Biological Functions and Engineering, Kyushu Institute of Technology, Kitakyushu 808-0196, Japan
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TANAKA Tomoya
Department of Biochemical Engineering, Graduate School of Science and Engineering, Yamagata University
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Tanaka Tomoya
Department of Biological Functions and Engineering, Kyushu Institute of Technology, Kitakyushu 808-0196, Japan
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Ikeno Shinya
Department of Biological Functions and Engineering, Kyushu Institute of Technology, Kitakyushu 808-0196, Japan
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Migita Satoshi
Department of Biological Functions and Engineering, Kyushu Institute of Technology, Kitakyushu 808-0196, Japan
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