Determination of the Expanded Optical Transfer Function in Saturated Excitation Imaging and High Harmonic Demodulation
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概要
- 論文の詳細を見る
Most limits of a microscope can be understood in terms of its spatial frequency mapping ability, known as the optical transfer function (OTF). Here, we demonstrate the expansion of the confocal fluorescence microscopy OTF under conditions where the population of excitation states of the sample is saturated. We show how the OTF of saturated excitation can be determined by estimating fluorescence signals using a five-level molecular electronic state model. We then show how modulating excitation and demodulating fluorescence signals can extract high frequency components. We provide experimental validation by demonstrating that harmonic demodulation can extract high frequency OTF components.
- 2011-04-25
著者
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Yamanaka Masahito
Department Of Urology University Of Tokushima School Of Medicine
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Fujita Katsumasa
Department Of Applied Physics Graduate School Of Engineering Osaka University
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Kawata Satoshi
Department Of Appkied Physicy Osaka University
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Smith Nicholas
Immunology Frontier Research Center, Osaka University, Suita, Osaka 565-0871, Japan
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Kawano Shogo
Department of Frontier Biosciences, Osaka University, Suita, Osaka 565-0871, Japan
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Kawata Satoshi
Department of Applied Physics, Osaka University, Suita, Osaka 565-0871, Japan
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Fujita Katsumasa
Department of Applied Physics, Osaka University, Suita, Osaka 565-0871, Japan
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Yamanaka Masahito
Department of Frontier Biosciences, Osaka University, Suita, Osaka 565-0871, Japan
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