Application of Poly[oxyethylene(dimethylimino)propyl-(dimethylimino)ethylene] as Enzyme Stabilizer for Bilirubin Oxidase Immobilized Electrode
スポンサーリンク
概要
- 論文の詳細を見る
It has been shown that polyammonium cations comprising quaternary ammonium and hydrophilic groups such as amide and hydroxyl groups stabilize a redox enzyme bilirubin oxidase (BOD). The BOD catalyzes the reaction: 4[Fe(CN)6]4− + 4H+ + O2 → 4[Fe(CN)6]3− + 2H2O, and has been a promising enzyme for use as a cathode catalyst in biofuel cells. In this study, the stabilizing effect of poly[oxyethylene(dimethylimino)propyl(dimethylimino)ethylene] (PA1) on BOD has been investigated. The sample solution containing BOD and the PA1 salt was kept at a given temperature, and the loss of the enzymatic activity was detected after given stored times. The activity decreased exponentially with stored time so that the first-order rate-constant of inactivation was determined. The inactivation rate-constant lowered with increasing the concentration of the PA1 salt, suggesting that BOD was stabilized by the association with the PA1 cation. The PA1 cation may act like a protective colloid or decrease the local disorder of BOD by its wrapping. A membrane-covered electrode containing BOD, PA1, and [Fe(CN)6]4−/3− in the internal solution phase was examined in air-saturated aqueous solution. The electrode gave a well-defined current-potential curve with a steady state limiting current due to the PA1-[Fe(CN)6]4−/3− polyion complex-mediated bioelectrocatalytic current for the reduction of O2. The decreasing of the steady state limiting current became slower in the presence of the PA1 salt, indicating again the stabilizing effect of PA1 cation on BOD.
- 社団法人日本分析化学会の論文
社団法人日本分析化学会 | 論文
- 微生物学的試験法による畜産物中に残留する抗菌性物質の高感度測定法(安心と安全に役立つ分析化学)
- 液体クロマトグラフィー/質量分析法による缶飲料中のビスフェノールAの定量 (環境ホルモンと分析化学)
- 高速液体クロマトグラフィーによるL-シトルリン測定を指標としたマクロファージの一酸化窒素産生の評価 ( バイオサイエンスと分析化学)
- 小型定電流電解電量/光度滴定装置の開発と食用油の過酸化物価定量への応用
- 固定化ヒスタミンオキシダーゼを用いる吸光検出フローインジェクション分析による魚醤中のヒスタミンの定量(安心と安全に役立つ分析化学)