Identification of the 58-kDa Phosphoprotein Associated with Motility Initiation of Hamster Spermatozoa
スポンサーリンク
概要
- 論文の詳細を見る
In our previous paper [M. Fujinoki et al. (2001) Biomed. Res. 22, 45-58], we reported that the 58-kDa protein obtained from hamster sperm flagella was phosphorylated at serine residues in association with the start of motility. In the present experiments, we identified and localized the 58-kDa protein. The 58-kDa protein was assumed to exist in the acrosomal region domain of the sperm head and the whole sperm flagellum. In particular, a large amount of 58-kDa protein was localized in the equatorial segment of the acrosomal region domain of the sperm head and the middle piece of the sperm flagellum. In the next step, the 58-kDa protein was identified by peptide mass finger printing and LC-MS/MS analysis. The results suggested that the 58-kDa protein was ATP synthase H+ transporting F1 β, which is one of the mitochondrial components. Therefore, it is likely that the 58-kDa protein is associated with ATP production in the mitochondrial sheath in the middle piece of the sperm flagellum, and H+ transport in the sperm head and the sperm flagellum except for the middle piece, since ATP synthase also acts as an H+ pump.
- 社団法人 日本生化学会の論文
著者
-
Toda Toshifusa
TMIG Proteomics Collaboration Center, Tokyo Metropolitan Institute of Gerontology
-
Shimizu Nobuyoshi
Department Of Cancer And Thoracic Surgery Okayama University Graduate School Of Medicine
-
Fujinoki Masakatsu
Department Of Cellular And Humoral Physiology Dokkyo University School Of Medicine
-
Ohtake Hideki
Department Of Physiology Dokkyo University Of Tokyo
-
Yamaoka Sadao
Department Of Cellular And Humoral Physiology Dokkyo University School Of Medicine
-
Kawamura Takeshi
Department Of Biodefense Nagoya City University Graduate School Of Medical Sciences
-
Okuno Makoto
Department Of Biology College Of Arts And Sciences University Of Tokyo
関連論文
- The genome structure and the genomic comparison of a medaka hox cluster(Symposium on Comparative and Functional Genomics of Medaka)
- PJ-626 The anti-60kDa Heat-Shock Protein IgM Antibody as a Pathophygiological Candidate after the Repetitive Coxsackievirus Infection.(Cardiomyopathy, Basic and Clinical 2 (M) : PJ105)(Poster Session (Japanese))
- Inflammatory pseudotumor of the bladder in neonates
- Congenital vesticovaginal fistula
- CHARACTERIZATION OF THE MEDAKA Y-CHROMOSOME SPECIFIC REGION(Genetics,Abstracts of papers presented at the 76^ Annual Meeting of the Zoological Society of Japan)
- The DNA Sequence of Medaka Chromosome LG22(Comparative Genomics of Animals,Symposium,PROCEEDING OF THE 76^ ANNUAL MEETING OF THE ZOOLOGICAL SOCIETY OF JAPAN)
- POLYMORPHISM OF THE MAJOR HISTOCOMPATIBILITY COMPLEX CLASS I REGION OF MEDAKA, ORYZIAS LA TIPES(Taxonomy and Systematics,Abstracts of papers presented at the 75^ Annual Meeting of the Zoological Society of Japan)
- Genomic Structure and Expression of the Soluble Guanylyl Cyclase α2 Subunit Gene in the Medaka Fish Oryzias latipes
- Successful Treatment of Limited Disease-Small Cell Lung Cancer with Multimodality Treatment Consisting of Concurrent Chemoradiotherapy, High-Dose Chemotherapy with Autologous Peripheral Blood Stem Cell Transplantation and Surgical Resection
- Lymphoepithelioma-like carcinoma of the renal pelvis
- Inhibition of tracheal smooth muscle cell proliferation by phosphodiesterase inhibitors
- Accumulation of Identical T Cell Clones in the Right and Left Lobes of the Thyroid Gland in Patients with Graves' Disease : Analysis of T Cell Clonotype in vivo
- Correlation of clonal T cell expansion with disease activity in systemic lupus erythematosus
- Clonal prevalence of T cells infiltrating into the pancreas of prediabetic non-obese diabetic mice
- PROTEOMIC ANALYSIS OF MOTILITY ACTIVATION IN CIONA INTESTINALIS(Cell Biology and Morphology,Abstracts of papers presented at the 74^ Annual Meeting of the Zoological Society of Japan)
- Isolation and Characterization of the Plasma Hyaluronan-Binding Protein (PHBP) Gene (HABP2)
- Genomic Organization of the OS-9 Gene Amplified in Human Sarcomas
- Autoimmune Regulator (AIRE) Gene Is Expressed in Human Activated CD4^+ T-Cells and Regulated by Mitogen-Activated Protein Kinase Pathway
- Characterization of T Cell Receptor β Chains of Accumulating T Cells in Chronic Ongoing Myocarditis Demonstrated by Heterotopic Cardiac Transplantation in Mice
- Gene Analysis and Evaluation of the Single Founder Effect in Japanese Patients with Oguchi Disease
- Ex vivo Delivery of Suicide Genes into Melanoma Cells Using Epidermal Growth Factor Receptor-specific Fab Immunogene
- Construction of a BAC library for the red sea bream Pagrus major
- Fibroblast Growth Factor-2 Stimulates Interleukin-6 Secretion in Human Pancreatic Periacinar Myofibroblasts
- Autosomal recessive juvenile parkinsonism : A key to understanding nigral degeneration in sporadic Parkinson's disease
- Plasma Levels of Unactivated Thrombin Activatable Fibrinolysis Inhibitor (TAFI) Are Down-Regulated in Young Adult Women : Analysis of a Normal Japanese Population
- Modulation of Procarboxypeptidase R (ProCPR) Activation by Complementary Peptides to Thrombomodulin
- Important prognostic histological parameters for patients with invasive ductal carcinoma of the pancreas
- Construction of BAC libraries derived from the ascidian Ciona intestinalis
- Construction of a BAC library derived from the inbred Hd-rR strain of the teleost fish, Oryzias latipes
- CrossLinking of the CD69 Molecule Enhances S100A9 Production in Activated Neutrophils
- Proteomic Surveillance of Autoantigens in Relapsing Polychondritis
- Identification of Autoantibodies With the Corresponding Antigen for Repetitive Coxsackievirus Infection-Induced Cardiomyopathy
- 乳幼児の腎移植後の頭囲と発達の改善
- Light Dependent Induction of Early - Response Gene Exprossion by Calphostin - C
- A Single Cell Analysis of TCR AV24AJ18^+ DN T Cells
- Tissue specificity of tropomyosin isoform in the mussel, Mytilus galloprovincialis
- Intermittent Pneumatic Compression Is Effective in Preventing Symptomatic Pulmonary Embolism After Thoracic Surgery
- In situ Hybridization Histochemistry of c-erbA〆2 mRNA in the Hypothalamus and its Surrounding Structures in the Adult Male Rat
- Identification of 66 kDa phosphoprotein associated with motility initiation of hamster spermatozoa
- Anaplastic Thyroid Carcinoma Associated with Granulocyte Colony-Stimulating Factor : Report of a Case
- Transintestinal Systemic Oxygenation Using Perfluorocarbon
- Identification of the 58-kDa Phosphoprotein Associated with Motility Initiation of Hamster Spermatozoa
- Intraoperative Prediction of Compensatory Sweating for Thoracic Sympathectomy
- Transintestinal Oxygenation with Perfluorocarbon : Investigation of Perfusion Rate
- Identification of 36-kDa Flagellar Phosphoproteins Associated with Hamster Sperm Motility
- Long-term survival of a patient with advanced adenosquamous carcinoma of the gallbladder after radical resection
- Hemodynamic Effects of Pumpless Extracorporeal Membrane Oxygenation (ECMO) Support for Chronically Pressure-Overloaded Right Heart Failure in a Canine Experimental Model
- IDENTIFICATION AND LOCALIZATION OF 58 kDa PHOSPHOPROTEIN ASSOCIATED WITH THE MOTILITY OF HAMSTER SPERM(Cell Biology and Morphology)(Proceedings of the Seventy-Third Annual Meeting of the Zoological Society of Japan)
- Non-small Cell Lung Cancer Coexisting with Pulmonary Aspergilloma
- Rectal adenocarcinoma metastatic to the thyroid gland
- Allowable Warm Ischemic Time to Tracheal Extraction for Allotransplantation of Cryopreserved Trachea
- Living-Donor Lobar Lung Transplantation for End-Stage Primary Pulmonary Hypertension
- The Effects of Recombinant Tissue-Type Plasminogen Activator (rt-PA) on Canine Cadaver Lung Transplantation
- A Case of Thoracoscopic Bilateral Lung Volume Reduction Surgery in a Supine Position
- The genome size evolution of medaka (Oryzias latipes) and fugu (Takifugu rubripes)
- Thymidine Phosphorylase and Dihydropyrimidine Dehydrogenase Expression Levels in Tumor and Normal Tissue Specimens of T3 Human Colorectal Carcinoma
- Chimeric Human/Murine Monoclonal IgM Antibodies to HIV-1 Nef Antigen Expressed on Chronically Infected Cells
- Effect of B-Ions Substitution in [(K_Bi_)-(Na_Bi_)](Ti-B)O_3 System with B=Zr, Fe_Nb_, Zn_Nb_ or Mg_Nb_
- Serine/threonine phosphorylation associated with hamster sperm hyperactivation
- Expressoin of Prolactin Gene in Human Decidua During Pregnancy Studied by In Situ Hybrization Histochemistry
- Genomic Bacterial Artificial Chromosome Library of the Japanese Flounder Paralichthys olivaceus
- A-13 Expression of c-fos and c-jun protooncogens in the uteri of immature mice neonatally exposed to estrogen.
- c-Fos and c-Jun Expession in the Uteri of Neonatally Estrogen Exposed Mice
- Experimental Evaluation of the Effects of the Intraportal Administration of Cyclic Guanosine Monophosphate on Ischemia/Reperfusion in the Porcine Liver
- M-ISY-2 Improved Survival after Living-Donor Lobar Lung Transplantation
- Assignment of the human poly(A) polymerase (PAP) gene to chromosome 14q32.1-q32.2 and isolation of a polymorphic CA repeat sequence
- Regulation of hyperactivation of hamster spermatozoa by progesterone
- Effects of dibromoacetic acid on murine spermatozoa and testis
- SOLUBILIZATION OF SPERM FLAGELLAR PROTEINS FOR PROTEOME ANALYSIS(Cell Biology and Morphology,Abstracts of papers presented at the 74^ Annual Meeting of the Zoological Society of Japan)
- Changes of tyrosine phosphorylation associated with activation and hyperactivation in hamster sperm motility(Cell Biology and Morphology)Proceedings of the Seventy-First Annual Meeting of the Zoological Society of Japan
- PLC and PI3K Pathways are Important in the Inhibition of EGF-Induced Cell Migration by Gefitinib ('Iressa', ZD1839)
- Less Invasive Surgery for Primary Hyperparathyroidism Based on Preoperative ^Tc-hexakis-2-methoxyisobutylisonitrile Imaging Findings
- Identification of an HLA-A24-Restricted OY-TES-1 Epitope Recognized by Cytotoxic T-Cells
- Hepatitis Induced by an IgM Monoclonal Antibody against Procarboxypeptidase R
- Elastase from Activated Human Neutrophils Activates Procarboxypeptidase R
- PROTEIN KINASE C ACTIVITY AND PROTEIN PHOSPHORYLATION IN MOUSE SPERM
- Caveolin-1 as Tumor Suppressor Gene in Breast Cancer
- Isolation of a Gene for a Metallothionein-Like Protein from Soybean
- Profiling of proteins phosphorylated or dephosphorylated during hyperactivation via activation on hamster spermatozoa
- HUMAN GENOME PROJECT : CURRENT STATUS, KEIO STRATEGY AND PROSPECTS
- Angiogenesis Inhibitor TNP-470 Can Suppress Hepatocellular Carcinoma Growth Without Retarding Liver Regeneration After Partial Hepatectomy
- K^+ INDEPENDENT MOTILITY INITIATION IN CHUM SALMON SPERM TREATED WITH ORGANIC ALCOHOL, GLYCEROL(Cell Biology and Morphology,Abstracts of papers presented at the 76^ Annual Meeting of the Zoological Society of Japan)
- Construction of BAC library for the amphibian Xenopus tropicalis
- Approach to treatment of acute respiratory failure with liquid ventilation
- Screening of H-ras Gene Point Mutations in 50 Cases of Bladder Carcinoma
- Sperm-activating proteins from unfertilized eggs of the Pacific herring, Clrpea pallasii
- Adenovirus-mediated gene transduction of truncated IκBα enhances radiosensitivity in human colon cancer cells
- Isolation and characterization of antibodies against three consecutive Tn-antigen clusters from a phage library displaying human single-chain variable fragments
- PROTEIN DIFFERENTIAL DISPLAY FOR SPERM HYPERACTIVATION BY MEANS OF SELDI PROTEIN CHIP SYSTEM^[○!R](Cell Biology and Morphology,Abstracts of papers presented at the 76^ Annual Meeting of the Zoological Society of Japan)
- The Relationship between Sleep Circadian Rhythm and Central Feedback Mechanism of Gonadal Steroid in Female Guinea Pigs
- Non-genomic regulation of mammalian sperm hyperactivation
- PHOSPHOPROTEOME FOR MAMMALIAN SPERM CAPACITATION(Cell Biology and Morphology,Abstracts of papers presented at the 75^ Annual Meeting of the Zoological Society of Japan)
- Purification and characterization of prolyl endopeptidase from the Pacific herring, Clupea pallasi, and its role in the activation of sperm motility
- MutationView/KMcancerDB : A database for cancer gene mutations
- BREAST THERMOGRAPHY AND PROSTAGLANDIN E
- Targeted gene delivery using humanized single-chain antibody with negatively charged oligopeptide tail
- Cytoskeletal Active Drugs Modulate Signal Transduction in the Protein Kinase C Pathway
- COT-1 BANDING OF HUMAN CHROMOSOMES USING FLUORESCENCE IN SITU HYBRIDIZATION WITH CY3 LABELING
- Clinical and Angiographic Analysis of Congenital Coronary Artery Fistulae in Adulthood. Is There Any New Trend?:Is There Any New Trend?
- Identification of the 58-kDa Phosphoprotein Associated with Motility Initiation of Hamster Spermatozoa