Characterization of T Cell Receptor β Chains of Accumulating T Cells in Chronic Ongoing Myocarditis Demonstrated by Heterotopic Cardiac Transplantation in Mice
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概要
- 論文の詳細を見る
Autoimmne mechanisms have been implicated in the pathogenesis of chronic ongoing mycarditis. An earlier study of murine chronic ongoing myocarditis reported that infiltrating T cells and macrophages were prominent in the normal donor heart, in a heterotopic cardiac transplantation model. It was demonstrated that myocarditis was transferred to a normal heart transplanted into a mouse with chronic myocarditis. The present study investigated an autoimmune link to the pathogenesis of chronic ongoing myocarditis by analyzing the T cell clonalities in the model. To characterize the accumulating T cells in the donor heart, the T cell receptor β genes (TCRBG) were amplified by reverse transcriptase-polymerase chain reaction(RT-PCR) from mRNA in the donor hearts and accumulating TCRBG clonotypes were contrasted with those from recipient hearts. Inbred 3-week-old A/J mice were inoculated intraperitoneally with Coxsackievirus B3 (Nancy strain), 2×10^4 PFU, and housed for more than 60 days. Normal A/J mouse hearts were transplanted into the same strain of mice without myocarditis, as well as into the mice with chronic ongoing myocarditis. Both recipient and donor hearts were evaluated histologically 2 weeks after the transplantation. TCRBG were amplified by RT-PCR from mRNA of recipient and donor hearts and spleens. The specific accumulating TCRBG clonotypes were identified by their single strand conformation polymorphism. Multiple clonotypic accumulations occurred in the donor heart after cardiac transplantation. Distinct oligoclonal accumulation of TCR Vβl, 10, and 13 T cells was found in both recipient and donor hearts in 3 of 4 mice. Moreover, these clonotypes were not observed in spleen cells of the recipient mice. T specific cells expanding clonotypes of TCRBG are responsible for transferring myocarditis to the donor heart. An autoimmune response may, therefore, play a key role in the progression of chronic ongoing myocarditis.
- 社団法人日本循環器学会の論文
- 2001-01-20
著者
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Matsuzaki Masunori
The Department of Cardiovascular Medicine, Yamaguchi University Graduate School of Medicine
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Umemoto Seiji
Pharmaceutical Clinical Research Center Yamaguchi University Hospital
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Kato Tomohiro
Department Of Bioregulation And Proteomics Institute Of Medical Science St. Marianna University Scho
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KATO Tomohiro
Division of Rheumatology and Molecular Immunology, Institute of Medical Science, St Marianna Univers
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NISHIOKA Kusuki
Division of Rheumatology and Molecular Immunology, Institute of Medical Science, St Marianna Univers
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Yamamoto Takuo
The Department Of Medical Bioregulation Division Of Cardiovascular Medicine Yamaguchi University Sch
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Yamamura Taisei
The Department Of Medical Bioregulation Division Of Cardiovascular Medicine Yamaguchi University Sch
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Yamamura Taisei
Second Department Of Medicine Yamaguchi University
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Umemoto Seiji
The Department Of Medical Bioregulation Division Of Cardiovascular Medicine Yamaguchi University Sch
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Umemoto Seiji
Second Dept.of Int.med. Yamaguchi University School Of Medicine
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Nakamura Hiroshi
The Department of Medical Bioregulation, Division of Cardiovascular Medicine, Yamaguchi University S
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Sekine Taichi
Division of Rheumatology, Immunology and Genetics Program, Institute of Medical Science, St Marianna
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Umemoto Seiji
Yamaguchi National Hospital and Internal Medicine Yamaguchi Univ. School of Medicine
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Tsutsumi Kouichiro
Department Of Otorhinolaryngology St. Marianna University School Of Medicine
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Nishioka Kusuki
Department Of Bioregulation Institute Of Medical Science St. Marianna University School Of Medicine
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Sekine Taichi
Department Of Bioregulation Institute Of Medical Science St. Marianna University School Of Medicine
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Kawamura Takeshi
Department Of Biodefense Nagoya City University Graduate School Of Medical Sciences
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Kato T
Department Of Otorhinolaryngology St. Marianna University School Of Medicine
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Nakamura Hiroshi
Division Of Cardiology Yamaguchi University Graduate School Of Medicine
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Nakamura Hiroshi
The Department Of Medical Bioregulation Division Of Cardiovascular Medicine Yamaguchi University Sch
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Matsuzaki Masunori
The Depart. Of Int. Med. Yamaguchi Univers. School Of Med.
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