Construction of BAC library for the amphibian Xenopus tropicalis
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概要
- 論文の詳細を見る
Xenopus tropicalis has become an alternative model to the amphibian Xenopus laevis because it is better suited for genetic and genomic studies. We have constructed a genomic BAC library consisting of over 100,000 clones from sperm of Xenopus tropicalis. Analysis by pulsed field gel electrophoresis of representative BAC clones indicated the average size of insert DNA to be 100 kb, and we estimated the library covers 6 times the Xenopus tropicalis genome of 1.7 × 109 base pairs. To evaluate the BAC library, we attempted to isolate BAC clones which contain a protocadherin g (Pcdhg) gene and found that the isolated BAC clones are assembled as two separate contigs. This result suggests the presence of at least two clusters for the Pcdhg gene in the genome of X. tropicalis.
- 日本遺伝学会の論文
- 2004-02-25
著者
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Asakawa Shuichi
Department of Aquatic Biosciences, Graduate School of Agricultural and Life Sciences, The University
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Asakawa Shuichi
Department Of Aquatic Biosciences Graduate School Of Agricultural And Life Sciences The University O
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Shimizu Nobuyoshi
Department of Molecular Biology, School of Medicine, Keio University
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Asakawa Shuichi
Department Of Molecula Biology Keio University School Of Medicine
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ISHII Yasuyuki
Department of Physics, Faculty of Science, Saitama University
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Taguchi Yusuke
Kokoro Biology Group Laboratories For Integrated Biology Graduate School Of Frontier Biosciences Osa
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Shimizu Nobuyoshi
Department Of Cancer And Thoracic Surgery Okayama University Graduate School Of Medicine
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Ishii Yasuyuki
Department Of By Preclinical Dmpk Tsukuba Research Institute Banyu Pharmaceutical Co. Ltd
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YAGI Takeshi
KOKORO Biology Group, Laboratories for Integrated Biology, Graduate School of Frontier Biosciences,
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ISHIBASHI Shoko
Graduate School of Biological Sciences, Nara Institute of Science and Technology (NAIST)
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Yagi Takeshi
Kokoro Biology Group Laboratories For Integrated Biology Graduate School Of Frontier Biosciences Osa
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Shimizu Nobuyoshi
Department Of Molecula Biology Keio University School Of Medicine
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Ishibashi Shoko
Graduate School Of Biological Sciences Nara Institute Of Science And Technology (naist)
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Ishii Yasuyuki
Department Of Molecula Biology Keio University School Of Medicine
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Yagi Takeshi
Kokoro Biology Group Laboratories For Integrated Biology Graduate School Of Frontier Biosciences Osa
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Asakawa Shuichi
Department Of Aquatic Bioscience Graduate School Of Agricultural And Life Sciences The University Of
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Ishii Yasuyuki
Department Of Animal And Grassland Science Faculty Of Agriculture University Of Miyazaki
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Ishii Yasuyuki
Department of Advanced Radiation Technology, TIARA, JAEA, Takasaki, Gunma 370-1292, Japan
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