Effect of Aggregation of Amphotericin B on Lysophosphatidylcholine Micelles as Related to Its Complex Formation with Cholesterol or Ergosterol.

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概要

• 論文の詳細を見る

• The effect of aggregation of amphotericin B (AmB), as well as the complex formation of AmB with cholesterol or ergosterol, was investigated in micelles and vesicles. AmB in lysophosphatidylcholine (LPC) micelles adopted a more favorable monomeric form than that in other drug formulations. At an LPC/AmB ratio of 200, AmB existed only in monomeric form. Such monomeric behavior is likely dependent upon the fluidity and size of the micelles. In LPC micelles composed of 90% monomeric AmB, AmB-ergosterol complex formation occurred with an increase in the sterol concentration, but the complex formation of AmB-cholesterol was slight. On the other hand, in LPC micelles composed of 40% monomeric AmB, the complex formation of AmB-cholesterol as well as AmB-ergosterol was extensive. These results suggest that the complex formation of AmB with both sterols is highly dependent upon the aggregated state of AmB. In addition, using monolayers, mixtures of AmB/LPC/ergosterol were became more stable with rising temperature, while the stability of mixtures of AmB/LPC/cholesterol remained unchanged, implying that complex formation of AmB with cholesterol is different from that of AmB with ergosterol.

• 社団法人 日本生化学会の論文

著者

• Onda Maki

  Department Of Biological Science Graduate School Of Science Osaka Prefecture University

• KAWABATA Manami

  Department of Environmental Sciences, Faculty of Science, Osaka Women's University

• Mita Tomoyoshi

  Department Of Applied Microbial Technology The Kumamoto Institute Of Technology

関連論文

• Susceptibilities of Phospholipid Vesicles Containing Different Sterols to Amphotericin B-Loaded Lysophosphatidylcholine Micelles
• Effect of Aggregation of Amphotericin B on Lysophosphatidylcholine Micelles as Related to Its Complex Formation with Cholesterol or Ergosterol^1
• Interaction of Gramicidin with Lysophosphatidyicholine as Revealed by Calorimetry and Fluorescence Spectroscopy
• Susceptibilities of Phospholipid Membranes Containing Cholesterol or Ergosterol to Gramicidin and its Derivative Incorporated in Lysophospholipid Micelles
• Effect of Aging on the Rheological Properties of Gluten Gel(Food & Nutrition)
• Interaction of Phospholipid and Antibiotic Ionophores, Gramicidin A' and Valinomycin, in Mixed Monolayers
• Conformation of Gramicidin in Monolayers, Organic Solvents and Phospholipid Bilayers
• Interaction of Amphotericin B with Cholesterol in Monolayers, Aqueous Solutions, and Phospholipid Bilayers
• Lipid-protein interaction in mixed monolayers from phospholipids and proteins.
• Effect of Aggregation of Amphotericin B on Lysophosphatidylcholine Micelles as Related to Its Complex Formation with Cholesterol or Ergosterol.

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