Lipid-protein interaction in mixed monolayers from phospholipids and proteins.
スポンサーリンク
概要
- 論文の詳細を見る
The thermotropic behavior of different proteins with phospholipids in monolayers was investigated by measuring the surface pressure and the surface viscosity. When phospholipids, such as dipalmitoylphosphatidylcholine (DPPC) and dimyristoylphosphatidylcholine (DMPC), were mixed with the hydrophobic proteins, such as hemoglobin and β-casein, below their phase transition temperatures (<I>T</I><SUB>c</SUB>), a considerable expansion of monolayers was observed. On the other hand, when phospholipids were mixed with the hydrophilic proteins, such as bovine serum albumin (BSA) and lysozyme below <I>T</I><SUB>c</SUB>, no expansion was observed. When DMPC was mixed with different proteins at <I>T</I><SUB>c</SUB> or above, all mixtures formed slightly deviated monolayers from ideal mixing, independent of a variety of proteins. This type of behavior suggests that the hydrophobic proteins perturb the structure of boundary lipids near the protein molecule below <I>T</I><SUB>c</SUB>, while the hydrophobic proteins do not perturb the phospholipid structure below <I>T</I><SUB>c</SUB> because of their low hydrophobicities. At <I>T</I><SUB>c</SUB> or above the membrane properties are independent of a variety of proteins, because all lipid hydrocarbon chains cause a disordering, even without proteins. In addition, when DPPC was mixed with a hydrophobic protein, such as hemoglobin, the fluidity of monolayer increased significantly.
- 公益社団法人 日本化学会の論文
著者
関連論文
- Susceptibilities of Phospholipid Vesicles Containing Different Sterols to Amphotericin B-Loaded Lysophosphatidylcholine Micelles
- Effect of Aggregation of Amphotericin B on Lysophosphatidylcholine Micelles as Related to Its Complex Formation with Cholesterol or Ergosterol^1
- Interaction of Gramicidin with Lysophosphatidyicholine as Revealed by Calorimetry and Fluorescence Spectroscopy
- Susceptibilities of Phospholipid Membranes Containing Cholesterol or Ergosterol to Gramicidin and its Derivative Incorporated in Lysophospholipid Micelles
- Effect of Aging on the Rheological Properties of Gluten Gel(Food & Nutrition)
- Interaction of Phospholipid and Antibiotic Ionophores, Gramicidin A' and Valinomycin, in Mixed Monolayers
- Conformation of Gramicidin in Monolayers, Organic Solvents and Phospholipid Bilayers
- Interaction of Amphotericin B with Cholesterol in Monolayers, Aqueous Solutions, and Phospholipid Bilayers
- Lipid-protein interaction in mixed monolayers from phospholipids and proteins.
- Effect of Aggregation of Amphotericin B on Lysophosphatidylcholine Micelles as Related to Its Complex Formation with Cholesterol or Ergosterol.