Cloning of a Rat Glia Maturation Factor-.GAMMA. (rGMFG) cDNA and Expression of Its mRNA and Protein in Rat Organs.
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概要
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We isolated a rat glia maturation factor-γ (rGMFG) cDNA and examined the tissue distribution of GMFG in rat by Northern and Western blot analyses. Sequence analysis of the entire cDNA revealed an open reading frame of 426 nucleotides with a deduced protein of 142 amino acid residues. The deduced amino acid sequence of the putative product is highly homologous (78.9%) to rat glia maturation factor-β (rGMFB). Northern blot analysis indicated that a 0.9-kb mRNA is predominantly expressed in rat thymus, testis, and spleen. GMFG showed a different tissue distribution from GMFB, being present predominantly in proliferative and differentiative organs.
- 社団法人 日本生化学会の論文
著者
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Asai Kiyofumi
Department Of Bioregulation Research Nagoya City University Medical School
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Moriyama Akihiko
Division Of Biomolecular Science Institute Of Natural Sciences Nagoya City University
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KAWAI Yoko
Nagoya City University School of Nursing
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TADA Toyohiro
Nagoya City University School of Nursing
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Fujita Kaori
Department Of Otorhinolaryngology Caress Alliance Tenshi Hospital
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Inoue Yuichiro
Department Of Bioregulation Research Nagoya City University Medical School
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Yamamoto Manami
Department Of Bioregulation Research Nagoya City University Medical School
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Wada Yoshiro
Department Of Equilibrium Adaptation Research Division Of Higher Nervous Control Research Institute
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Tsuiki Hideki
Department Of Pediatrics And Neonatology Nagoya City University Graduate School Of Medical Sciences
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KATO Taiji
Department of Biochemistry, Nagoya City University Medical School
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