Changes of Tight Junction Protein Claudins in Small Intestine and Kidney Tissues of Mice Fed a DDC Diet
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概要
- 論文の詳細を見る
DDC (3,5-diethoxycarbonyl-1,4-dihydrocollidine)-fed mice are widely used as a model for cholestatic liver disease. We examined the expression of tight junction protein claudin subspecies by immunofluorescent histochemistry in small intestine and kidney tissues of mice fed a DDC diet for 12 weeks. In the small intestine, decreases in claudin-3, claudin-7 and claudin-15 were observed in villous epithelial cells corresponding to the severity of histological changes while leaving the abundance of these claudin subspecies unchanged in crypt cells. Nevertheless, the proliferative activity of intestinal crypt cells measured by immunohistochemistry for Ki-67 decreased in the mice fed the DDC diet compared with that of control mice. These results suggest the possibility that DDC feeding affects the barrier function of villous epithelial cells and thus inhibits the proliferative activity of crypt epithelial cells. On the other hand, in the kidney, remarkable changes were found in the subcellular localization of claudin subspecies in a segment-specific manner, although histological changes of renal epithelial cells were quite minimal. These results indicate that immunohistochemistry for claudin subspecies can serve as a useful tool for detecting minute functional alterations of intestinal and renal epithelial cells.
- 日本毒性病理学会の論文
著者
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Murata Masaki
Department Of Chemistry School Of Science The University Of Tokyo
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TAKEUCHI Masaya
Sapporo General Pathology Laboratory Co., LTD.
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Mori Michio
Sapporo Medical University School Of Medicine
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Kojima Takashi
Department Of Applied Chemistry And Biotechnology Chiba University
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Sawada Norimasa
Department Of Pathology
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Sawada Norimasa
Department of Pathology, Sapporo Medical University School of Medicine, S1 W17, Sapporo 060-8556, Japan
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Abiko Yukie
Sapporo General Pathology Laboratory Co., Ltd., 3-17, S12 W18, Sapporo 064-0912, Japan
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Tsujiwaki Mitsuhiro
Department of Pathology, Sapporo Medical University School of Medicine, S1 W17, Sapporo 060-8556, Japan
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Murata Masaki
Department of Pathology, Sapporo Medical University School of Medicine, S1 W17, Sapporo 060-8556, Japan
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Mori Michio
Sapporo General Pathology Laboratory Co., Ltd., 3-17, S12 W18, Sapporo 064-0912, Japan
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Takeuchi Masaya
Sapporo General Pathology Laboratory Co., Ltd., 3-17, S12 W18, Sapporo 064-0912, Japan
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