Recovery of mRNA Expression of Tryptophan 2,3-Dioxygenase and Serine Dehydratase in Long-Term Cultures of Primary Rat Hepatocytes.
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概要
- 論文の詳細を見る
Expression of tryptophan 2, 3-dioxygenase (TO) and serine dehydratase (SDH) has not previously been maintained or re-induced in long-term cultured hepatocytes. In the present study, we succeeded in inducing expression of TO and SDH mRNAs in adult rat hepatocytes cultured in serum-free L-15 medium supplemented with epidermal growth factor and 2% dimethyl sulfoxide (DMSO). After the start of culture, the expression of TO mRNA rapidly disappeared and at 96 h it was less than 10% of that at isolation. However, after the addition of 2% DMSO from 96 h, the transcript level gradually increased and reached about 40% of that of the isolated cells at day 14. In addition, the expression of TO mRNA was enhanced in cells treated with both 10-5M dexamethasone and 10-7M glucagon. In contrast, the expression of SDH mRNA decreased very rapidly and we could not detect it after 24 h of culture. Furthermore, 2% DMSO failed to induce it. However, when both 10-5M dexamethasone and 10-7M glucagon were added to the culture medium at day 9, we observed dramatic induction of SDH mRNA 24 h later. Primary hepatocytes cultured by this method could express and maintain highly differentiated hepatic functions for a long time. Thus, this in vitro system is suitable for the investigation of hepatic functions.
- 社団法人 日本生化学会の論文
著者
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Mitaka Toshihiro
Department Of Molecular Pathophysiology Cancer Research Institute Sapporo Medical University School
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Mochizuki Yohichi
Department Of Pathology Cancer Research Institute
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Nakamura Toshikazu
Biomedical Research Center Osaka University Medical School
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Kojima Takashi
Department Of Applied Chemistry And Biotechnology Chiba University
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Hirata Koichi
Department of Chemistry, Faculty of Science, Osaka University
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Mitaka Toshihiro
Department of Pathology, Cancer Research Institute
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Mizuguchi Torn
Department of Pathology, Cancer Research Institute
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