Effects of Cryopreservation on the Cell Viability, Proliferative Capacity and Neuronal Differentiation Potential of Canine Bone Marrow Stromal Cells

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概要

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• We investigated the cell viability, proliferative capacity and neuronal differentiation potential of canine bone marrow stromal cells (BMSCs) after cryopreservation. BMSCs were cryopreserved using cryoprotectant solutions with 10% DMSO and 10% FBS (DF group) or without DMSO and FBS (DF-free group); fresh BMSCs were used as a control. The cell viability and proliferative capacity of BMSCs were similar in the DF-free and control groups, while those in the DF group were lower. In all groups, BMSCs differentiated into neuron-like cells that stained positive against neuron markers, and the mRNA expression levels of neuron markers increased after neuronal induction. In conclusion, cryopreservation with DF-free cryoprotectant solution did not diminish the cell viability, proliferative capacity or neuronal differentiation potential of canine BMSCs.

著者

• TANAKA Shigeo

  Laboratory of Veterinary Surgery, Department of Veterinary Medicine, College of Bioresource Science,

• NAKANO Rei

  Laboratory of Veterinary Surgery, Department of Veterinary Medicine, College of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa, Kanagawa, 252–0880, Japan

• ASANO Kazushi

  Laboratories of Veterinary Surgery, Department of Veterinary Medicine, College of Bioresource Sciences, Nihon University

• TESHIMA Kenji

  Laboratories of Veterinary Surgery, Department of Veterinary Medicine, College of Bioresource Sciences, Nihon University

• EDAMURA Kazuya

  Laboratories of Veterinary Surgery, Department of Veterinary Medicine, College of Bioresource Sciences, Nihon University

• FUJIMOTO Kyohei

  Laboratory of Veterinary Surgery, Department of Veterinary Medicine, College of Bioresource Sciences, Nihon University

• TANAKA Shigeo

  Laboratory of Veterinary Surgery, College of Bioresource Sciences, Nihon University

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