Influence of an Autologous Serum-Supplemented Medium on the Proliferation and Differentiation into Neurons of Canine Bone Marrow Stromal Cells
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概要
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We investigated the influence of autologous serum (AS)-supplemented medium on the proliferation and differentiation into neurons of canine bone marrow stromal cells (BMSCs). Canine BMSCs were cultured using α-MEM only, α-MEM with 10% fetal bovine serum (FBS), and 5, 10 and 20% AS-supplemented α-MEM. Growth of canine BMSCs was observed in all AS groups. The proliferation capacity of canine BMSCs in the AS groups was similar to that in the FBS group. No significant differences between the FBS and AS groups were observed in the percentage of the cells that changed to the neuron-like morphology and neuron-specific enolase-positive ratio after neuronal differentiation. Canine BMSCs cultured using AS-supplemented medium were able to proliferate and showed neuronal differentiation potency.
著者
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NAKANO Rei
Laboratory of Veterinary Surgery, Department of Veterinary Medicine, College of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa, Kanagawa, 252–0880, Japan
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ASANO Kazushi
Laboratories of Veterinary Surgery, Department of Veterinary Medicine, College of Bioresource Sciences, Nihon University
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ASANO Kazushi
Laboratory of Veterinary Surgery, Department of Veterinary Medicine, College of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa, Kanagawa 252-0880, Japan
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KUROSAWA Takashi
Laboratory of Veterinary Surgery, Department of Veterinary Medicine, College of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa, Kanagawa 252-0880, Japan
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TESHIMA Kenji
Laboratories of Veterinary Surgery, Department of Veterinary Medicine, College of Bioresource Sciences, Nihon University
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TESHIMA Kenji
Laboratory of Veterinary Surgery, Department of Veterinary Medicine, College of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa, Kanagawa 252-0880, Japan
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EDAMURA Kazuya
Laboratories of Veterinary Surgery, Department of Veterinary Medicine, College of Bioresource Sciences, Nihon University
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TANAKA Shigeo
Laboratory of Veterinary Surgery, College of Bioresource Sciences, Nihon University
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TANAKA Shigeo
Laboratory of Veterinary Surgery, Department of Veterinary Medicine, College of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa, Kanagawa 252-0880, Japan
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NAKANO Rei
Laboratory of Veterinary Surgery, Department of Veterinary Medicine, College of Bioresource Sciences, Nihon University
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NAKANO Rei
Laboratory of Veterinary Surgery, Department of Veterinary Medicine, College of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa, Kanagawa 252-0880, Japan
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