Matrix Metalloproteinase-2 Degrades Fibrillin-1 and Fibrillin-2 of Oxytalan Fibers in the Human Eye and Periodontal Ligaments In Vitro

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概要

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• Oxytalan fibers are distributed in the eye and periodontal ligaments (PDL). The ciliary zonule, known as Zinn's zonule, in the eye is composed of oxytalan fibers, which are bundles of microfibrils consisting mainly of fibrillin-1 and fibrillin-2. As turnover of oxytalan fibers is slow during life, their degradation mechanism remains unclarified. This study was performed to examine degradation pattern of fibrillin-1 and fibrillin-2 by experimental MMP activation. We cultured human non-pigmented ciliary epithelial cells (HNPCEC) and PDL fibroblasts for 7 days, then treated them with concanavalin A to activate matrix metalloproteinase (MMP)-2, and examined the degradation of fibrillin-1 and fibrillin-2 for 72 hr using immunofluorescence. At 7 days of HNPCEC culture, fibrillin-1-positive fibers were observed, some of which merged with fibrillin-2. After MMP-2 activation, fibrillin-1-positive fibers became thin and disappeared by 72 hr, while fibrillin-2-positive fibers disappeared almost completely within 24 hr. At 7 days of PDL fibroblast culture, fibrillin-1-positive fibers were mostly merged with fibrillin-2. After MMP-2 activation, fibrillin-1-positive fibers became thin by 24 hr and had almost disappeared by 48 hr, while fibrillin-2-positive fibers decreased constantly after 24 hr. A MMP-2 inhibitor completely suppressed these degradations. These results suggest that the patterns of fibrillin-1 and fibrillin-2 degradation differ between the eye and the PDL, possibly reflecting the sensitivity of fibrillin-1 and fibrillin-2 of each type of oxytalan fiber against MMP-2.

• 日本組織細胞化学会の論文

著者

• Oka Kyoko

  Section Of Functional Structure Department Of Morphological Biology Division Of Biomedical Sciences

• Ishikawa Hiroyuki

  Section Of Orhodontics Department Of Oral Growth Development Division Of Clinical Dentistry Fukuoka

• Oka Kyoko

  Section of Pediatric Dentistry, Department of Oral Growth and Development, Division of Clinical Dentistry, Fukuoka Dental College

• Kawagoe Megumi

  Section of Orthodontics, Department of Oral Growth and Development, Division of Clinical Dentistry, Fukuoka Dental College

• Tsuruga Eichi

  Section of Functional Structure, Department of Morphological Biology, Division of Biomedical Sciences, Fukuoka Dental College

• Sawa Yoshihiko

  Section of Functional Structure, Department of Morphological Biology, Division of Biomedical Sciences, Fukuoka Dental College

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