Studies on Insulin and Anti-Insulin Antibody Levels in Sera of Insulin-Treated Patients:2) Evaluations of Anti-Insulin Antibody Index (Sebriakova), Antibody-Bound Insulin, and 125I-Insulin Binding Ratio for the Determination of Anti-Insulin Ant

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Anti-insulin antibody index (Sebriakova), antibody-bound insulin, and 125I-insulin binding ratio were examined to determine anti-insulin antibody. (1) Anti-insulin antibody index (sebriakova) gave good correlation with low-affinity insulinbinding capacity (Scatchard analysis)(r=0.89). It also correlated with antibody-bound insulin concentration (r=0.71). (2) Antibody-bound insulin concentration gave good correlation with high-affinity insulinbinding capacity (Scatchard analysis)(r=0.73). (3) 125I-insulin binding ratio correlated poorly with low-or high-affinity insulin-binding capacity (Scatchard analysis), antibody-bound insulin, or anti-insulin antibody index (Sebriakova). But for 125I-insulin binding ratios up to 50%, better correlation was obtained between 125I-insulin binding ratio and antibody-bound insulin or anti-insulin antiboy index (Sebriakova). These results suggest that anti-insulin antibody index (Sebriakova) and antibody-bound insulin are useful to ascertain low-affinity insulin binding capacity, and high-affinity insulinbinding capacity, respectively.
Japan Society of Clinical Chemistryの論文