酸素耐性直接電子移動型亜酸化窒素還元酵素を用いた亜酸化窒素センシング
スポンサーリンク
概要
- 論文の詳細を見る
Nitrous oxide (N2O) is known as a greenhouse gas and a dominant ozone-depleting substance. It is is released mainly from agricultural processes. Therefore, the development of an on-site monitoring system is required to measure N2O concentration and control the release from the source. Using oxygen-insensitive cytochrome c-type nitrous oxide reductase, wNosZ, from Wolinella succinogenes, we have developed an electrochemical enzyme sensor. The sensor signal depends on the concentration of N2O in a reaction cell containing methoxy-5-methylphenazinium methylsulfate (mPMS) as an electron mediator under Ar atmosphere and, surprisingly, under air. Moreover, in the absence of addition of an electron mediator to the reaction cell, we observe that the reduction current depends on the concentration of N2O, which implies direct electron transfer. The wNosZ electrode is stable when stored at 4°C for 2 weeks and is specific to N2O. These results suggest that wNosZ holds great promise as a component of a novel direct-electron-transfer-type electrochemical sensing system for N2O.
著者
-
Tsugawa Wakako
Department Of Biotechnology And Life Science Faculty Of Engineering Tokyo University Of Agriculture
-
Ueno Yoshiyuki
Kajima Technical Research Institute
-
Tatara Masahiro
Kajima Technical Research Institute
-
Sode Koji
Department Of Biotechnology And Life Science Faculty Of Engineering Tokyo University Of Agriculture
-
SHIMIZU Hitomi
Department of Biotechnology, Graduate School of Engineering, Tokyo University of Agriculture & Technology
-
SODE Koji
Department of Biotechnology, Graduate School of Engineering, Tokyo University of Agriculture and Technology
-
KOJIMA Katsuhiro
Department of Biotechnology, Graduate School of Engineering, Tokyo University of Agriculture and Technology
-
TSUGAWA Wakako
Department of Biotechnology, Graduate School of Engineering, Tokyo University of Agriculture and Technology
-
KOJIMA Katsuhiro
Department of Biotechnology, Graduate School of Engineering, Tokyo University of Agriculture & Technology
関連論文
- ホスホトリエステラーゼの活性中心を模倣した構造を持つポリマーを用いた有機リン系殺虫剤測定用新規アンペロメトリックセンサの構築
- Wireless monitoring of blood glucose levels in flatfish with a needle biosensor
- 架橋によるガラクト-スオキシダ-ゼの基質特異性の改変
- 電導度検出に基づく有機リン系殺虫剤の新しい酵素分析法の開発
- 1,5-アンヒドロ-D-グルシト-ル高感度計測用海洋微生物センサの開発〔英文〕 (バイオエレクトロケミストリ-の新展開)
- Single Amino Acid Preferences for Specific Locations at Type-I α-Turns in Globular Proteins
- A New Type of Φ, Ψ Representation of the Protein Tertiary Structure and the Analysis of the Amino Acid Preferences for Speciffic Locations at Type-II β-Turn by Using 8000 Possible Kinds of Amino Acid Residues
- Statistical Characterization of Eleven Kinds of Helix Elements with Amino Acid Residues in the Middle of Triplets
- Archaeal Population on Supporting Material in Methanogenic Packed-Bed Reactor(ENVIRONMENTAL BIOTECHNOLOGY)
- Microbial Community in Methanogenic Packed-Bed Reactor Successfully Operating at Short Hydraulic Retention Time(ENVIRONMENTAL BIOTECHNOLOGY)
- Characterization of a Microorganism Isolated from the Effluent of Hydrogen Fermentation by Microflora
- Growth properties of a marine bacterium capable of assimilating 1,5-anhydro-D-glucitol as the sole carbon source
- Hydrogen Production from Industrial Wasterwater by Anaerobic Microflora in Chemostat Culture
- Biological Production of Hydrogen from Cellulose by National Anaerobic Microflora
- Phylogenetic Analyses of Bacterial Communities Developed in a Cassette-Electrode Microbial Fuel Cell
- Cloning and Expression of Fructosyl-amine Oxidase from Marine Yeast Pichia Species N1-1
- プルシャンブルーフィルムを用いたフルクトシルアミン酵素センサの開発
- Screening and Characterization of Fructosyl-Valine-Utilizing Marine Microorganisms
- Efficient Conversion of Dinitrile to Mononitrile-Monocarboxylic Acid by Corynebacteriu sp. C5 Cells during Tranexamic Acid Synthesis
- Methanogenic pathway and community structure in a thermophilic anaerobic digestion process of organic solid waste(ENVIRONMENTAL BIOTECHNOLOGY)
- Induction of a temperate marine cyanophage by heavy metal
- Recovery of a marine cyanobacterial recombinant product using fish-feed organisms
- Involvement of Tyrosine Hydroxylase Upregulation in Cyclosporine-Induced Hypertension
- Methanogenic pathway and community structure in a thermophilic anaerobic digestion process of organic solid waste
- Discrepancy between subjective and objective sleep in patients with depression
- EDTA耐性が向上したキメラPQQGDHの構築とキャラクタリゼ-ション
- グルコース脱水素酵素を用いた電気化学的SNP検出法の開発
- Biochemical Properties and Colony Formation Patterns of Salmonella-Like Strains
- 酸素耐性直接電子移動型亜酸化窒素還元酵素を用いた亜酸化窒素センシング
- 針型超小型電極を用いた直接電子移動型グルコースセンサ
- BioLC-Oscillator: A Self-Powered Wireless Glucose-Sensing System with the Glucose Dependent Resonance Frequency
- Sensitive Electrochemical ATP Assay Combined with Enzymatic ATP Amplification Reaction
- Candidatus Methanogranum caenicola: a Novel Methanogen from the Anaerobic Digested Sludge, and Proposal of Methanomassiliicoccaceae fam. nov. and Methanomassiliicoccales ord. nov., for a Methanogenic Lineage of the Class Thermoplasmata
- Operational Condition of a Molecular Imprinting Catalyst-based Fructosyl-valine Sensor
- Aggregation and Fibrillation Study of α-synuclein Under Applied Voltage
- Candidatus Methanogranum caenicola : a Novel Methanogen from the Anaerobic Digested Sludge, and Proposal of Methanomassiliicoccaceae fam. nov. and Methanomassiliicoccales ord. nov., for a Methanogenic Lineage of the Class Thermoplasmata
- Inhibitory Effects of Ferrihydrite on a Thermophilic Methanogenic Community