Sensitive Electrochemical ATP Assay Combined with Enzymatic ATP Amplification Reaction
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概要
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We propose an electrochemical, low-concentration adenosine triphosphate (ATP) assay combined with enzymatic ATP amplification. The electrochemical ATP assay consists of four parts: (i) myokinase for converting adenosine monophosphate (AMP) + ATP to two adenosine diphosphate (ADP) molecules, (ii) pyruvate kinase for converting two ADP molecules back to two ATP molecules (ATP amplification) and conversion of phosphoenolpyruvate to pyruvate, (iii) pyruvate dehydrogenase for generating H2O2 coupling with pyruvate oxidation, and (iv) electrochemical detection of H2O2. This system was used to detect ATP with a detection limit of 10−9 M ATP.
著者
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Tsugawa Wakako
Department Of Biotechnology And Life Science Faculty Of Engineering Tokyo University Of Agriculture
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KOJIMA Katsuhiro
Department of Biotechnology, Graduate School of Engineering, Tokyo University of Agriculture & Technology
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OKAMURA Daisuke
Panasonic healthcare Co. Ltd.
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MACHIDA Kunihiko
Panasonic healthcare Co. Ltd.
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KAWAHARA Takeshi
Panasonic healthcare Co. Ltd.
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