Studies on dissolved metalloenzymes in lake water. II Seasonal variations in phosphatase activity in lake kasumigaura.
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概要
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Alkaline phosphatase activity in Lake Kasumigaura was determined periodically and characterized by highperformance liquid chromatography and isoelectric focusing electrophoresis. There are two peaks in dissolved alkaline phosphatase activity; the bigger one in summer and the smaller one in winter. Molecular weight of ca. 89000 and isoelectric points 5–6 were found for most phosphatases in samples collected during the summer. A considerable part of the winter alkaline phosphatases, however, have much higher molecular weights, and major isoelectric points for these were ca. 4. These alkaline phosphatases were metalloenzymes activated by zinc. The observations above suggest that there are seasonal changes in the dominant microbial species producing dissolved alkaline phosphatase; i.e., blue-green algae, a procaryote, in summer and diatoms and flagellates, eucaryotes, in winter. The peak of alkaline phosphatase activity in lake water was predictive of a following increase in algal species therein.
- 公益社団法人 日本化学会の論文
著者
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Kobayashi Kensei
Department Of Chemistry And Biotechnology Faculty Of Engineering Yokohama National University
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Fujiwara Kitao
Department Of Chemistry Faculty Of Science University Of Tokyo
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Fuwa Keiichiro
Department Of Chemistry Faculty Of Science University Of Tokyo
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Hashimoto Shinya
Department Of Chemistry Faculty Of Science University Of Tokyo
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Haraguchi Hiroki
Department of Agricultural Chemistry, Faculty of Agriculture The University of Tokyo
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Hashimoto Shinya
Department of Chemistry, Faculty of Science, The University of Tokyo
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Iwase Koichi
Department of Chemistry, Faculty of Science, The University of Tokyo
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Otsuki Akira
National Institute for Environmental Studies
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Fuwa Keiichiro
Department of Agricultural Chemistry, Faculty of Agriculture The University of Tokyo
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